• 关键词：植物修复  cdr1-1突变  镉  拟南芥

• 基金项目：国家自然科学基金项目(No.30570145);合肥工业大学博士专项基金资助项目(No.035019)

• 曹树青
• 合肥工业大学生物与食品工程学院生物技术系, 合肥 230009

• 黄琼
• 合肥工业大学生物与食品工程学院生物技术系, 合肥 230009

• 魏鹏
• 合肥工业大学生物与食品工程学院生物技术系, 合肥 230009

• 摘要：从甲基磺酸乙酯(EMS)诱变获得的拟南芥M2代群体(Columbia型)中筛选获得一个耐受镉Cd²⁺毒害能力显著增强的拟南芥突变体(命名为cdr1-1).遗传分析表明,该突变性状为隐性单基因突变,与野生型相比,cdr1-1突变体在不同发育时期均能耐受Cd²⁺毒害,且其对Cd²⁺的积累能力也显著高于野生型.此外,还发现cdr1-1突变体体内的还原型谷胱甘肽(GSH)水平显著高于野生型,用GSH合成抑制剂丁硫氨酸亚矾胺处理cdr1-1突变体,导致其耐受Cd²⁺毒害能力显著下降,几乎接近野生型水平,表明cdr1-1突变体对Cd²⁺的耐受性至少部分依赖于GSH介导的途径.

• Abstract：An Arabidopsis mutant (designated cdr1-1) was identified that had enhanced tolerance to cadmium (Cd²⁺) toxicity by screening ethyl methanesulfonate-mutagenized Arabidopsis M2 seedlings (ecotype Columbia). Genetic analysis indicated that the cdr1-1 mutation was recessive and segregated as a single gene. At different development stages, cdr1-1 showed enhanced tolerance to Cd²⁺ toxicity compared with the wild type. Also, a higher Cd²⁺ accumulation was detected in cdr1-1 seedlings than that in wild-type seedlings. In addition, it was also found that glutathion (GSH) content was enhanced significantly in cdr1-1plants. Treatment with BSO, an inhibitor of GSH biosynthesis, resulted in a significantly decreased tolerance of the cdr1-1 mutant to Cd²⁺ toxicity, which was comparable to that of the wild type, suggesting that the enhanced tolerance of cdr1-1 to Cd²⁺toxicity is dependent, at least in part, on a GSH-medicated pathway.

• Key words：Phytoremediation  cdr1-1 mutation  cadmium  Arabidopsis

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