污染控制技术及原理
周晓燕,文湘华,冯嫣.葡萄糖补料对白腐真菌P.chrysosporium产木质素降解酶的影响[J].环境科学学报,2007,27(3):363-368
葡萄糖补料对白腐真菌P.chrysosporium产木质素降解酶的影响
- The influence of glucose feeding on the ligninolytic enzymes production of the white-rot fungus P.chrysosporium
- 基金项目：国家自然科学基金项目(No.20577028)
- 作者
- 单位
- 周晓燕
- 环境模拟与污染控制国家重点实验室, 清华大学环境科学与工程系, 北京 100084
- 文湘华
- 环境模拟与污染控制国家重点实验室, 清华大学环境科学与工程系, 北京 100084
- 冯嫣
- 环境模拟与污染控制国家重点实验室, 清华大学环境科学与工程系, 北京 100084
- 摘要：为了提高木质素降解酶的产量,采用氮限制培养基(C/N=56.0/8.8),研究了葡萄糖补料对P.chrysosporium产木质素降解酶的影响.结果表明,在培养第1d即接种24h后进行葡萄糖补料,可以刺激菌丝体的生长和产酶,补料浓度达到2g·L-1时,补料体系中的MnP酶活可提高至空白对照样(不进行补料)的2.5倍.同时,对葡萄糖分批补料方式进行的试验结果显示,培养过程中每24h进行1次补料,葡萄糖补料终浓度为1.5g·L-1的补料方式,与每48h的补料方式相比,产酶效果更好.MnP酶活峰值和达到峰值时酶的总产量可提高至空白对照样(不进行补料)的2.7倍和3.0倍,且酶活能够在200U·L-1以上稳定4d.实验结果说明,葡萄糖补料可以有效刺激白腐真菌产酶,且连续低浓度葡萄糖补料可获得较优的产酶效果.
- Abstract：To increase the production of ligninolytic enzymes, the present work studied the influence of glucose feeding on ligninolytic enzyme production of P.chrysosporium in nitrogen limited (C/Nratio is 56.0/8.8) medium. The result showed that 2g·L-1 glucose addition on the 1st day of the culture (24h after the inoculation) could stimulate fungal biomass growth and enzymes production. The MnPactivity was 2.5 times greater than those resulted from cultures without glucose feeding. Furthermore, the glucose feeding mode in fed-batch culture was tested. Compared with glucose feeding of every 48h, cultures with glucose feeding of every 24h and with a final concentration of 1.5g·L-1 produced higher enzyme activity. The peak and yield of MnPactivity was 2.7 and 3.0 times greater compared to the contrast culture, and it could keep stable for 4 days with an activity over 200U·L-1.
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