污染控制技术及原理
王海燕,周岳溪,戴欣,柴延丽,蒋进元,刘双江.16SrDNA克隆文库方法分析MDAT-IAT同步脱氮除磷系统细菌多样性研究[J].环境科学学报,2006,26(6):903-911
16SrDNA克隆文库方法分析MDAT-IAT同步脱氮除磷系统细菌多样性研究
- Bacterial diversity study for the simultaneous nitrogen and phosphorus removal system (MDAT-IAT) by 16S rDNA cloning method
- 基金项目：国家"863"计划(No.2002AA601320B)
- 作者
- 单位
- 王海燕
- 中国环境科学研究院, 北京 100012
- 周岳溪
- 中国环境科学研究院, 北京 100012
- 戴欣
- 中国科学院微生物研究所, 北京 100080
- 柴延丽
- 中国环境科学研究院, 北京 100012
- 蒋进元
- 中国环境科学研究院, 北京 100012
- 刘双江
- 中国科学院微生物研究所, 北京 100080
- 摘要：采用分子生物学手段16SrDNA克隆文库方法对高效同步脱氮除磷系统MDATIAT(modified demand aeration tank-intermit aeration tank)的IAT池中的细菌进行了多样性研究.从16SrDNA克隆文库中随机挑选59个克隆子进行序列测定(约500bp),对测序结果进行了BLAST比对.结果表明,MDATIAT系统中IAT池的细菌群落具有高度多样性,有55个克隆子分属6个不同的细菌类群,3个克隆子属于未知类群,优势细菌类群为Proteobacteria类群(变形菌类群),占55.17%;细菌类群优势顺序为γ-Proteobacteria类群(34.48%)、Bacteroidetes类群(似杆菌类群,20.69%)、β-Proteobacteria类群(12.07%)、Candidate division TM7类群(12.07%)、α-Proteobacteria类群(5.17%)、δ-Proteobacteria类群(3.45%)、Firmicutes类群(厚壁菌类群,3.45%)、Candidate division OP11类群(1.72%)、Planctomycetes类群(浮霉状菌类群,1.72%).用clustalx软件从测序的58个克隆子中选出32个OTU进行系统发育分析,同样表明IAT池的细菌多样性较强.
- Abstract：The bacterial diversity in the IAT tank of the Modified Demand Aeration Tank-Intermit Aeration Tank system (MDAT-IAT ), which was of high efficiency for the simultaneous nitrogen and phosphorus removal, was studied by the 16S rDNA cloning and sequencing method. 59 clones were randomly selected, and their partial 16S rDNA gene (ca. 500 bp) was sequenced and blasted. The results indicated that the bacterial diversity in the IAT tank of the MDAT-IAT was very high, where 55 clones belong to 6 different published phyla, while 3 clones belong to unknown phylum. The dominant bacterial community in the IAT tank was Proteobacteria, which accounted for 55.17 %. The bacterial community succession were as follows: the γ-Proteobacteria (34.48%),Bacteroidetes (20.69%), β-Proteobacteria (12.07%), Candidate division TM7 (12.07%), α-Proteobacteria (5.17%), Proteobacteria (3.45%), Firmicute (3.45%), Candidate division OP11 (1.72%), Planctomycetes (1.72%). 32 OUT phylotypes were selected from the 58 sequenced clones by Clustralx Software, and the phylogenetic analysis results also demonstrated high bacterial diversity in the IAT tank.
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