污染控制技术及原理
徐昕荣,贾晓珊,陈杰娥.一种未见报道过的厌氧氨氧化微生物的鉴定及其活性分析[J].环境科学学报,2006,26(6):912-918
一种未见报道过的厌氧氨氧化微生物的鉴定及其活性分析
- Identification and substrate degradation of a novel type of anammox bacterium
- 基金项目："985"工程二期项目;中山大学重点资助项目(No.3200023253282)
- 作者
- 单位
- 徐昕荣
- 中山大学环境科学与工程学院, 广州 510275
- 贾晓珊
- 中山大学环境科学与工程学院, 广州 510275
- 陈杰娥
- 中山大学环境科学与工程学院, 广州 510275
- 摘要：采用完全混合连续流反应器,以硝化污泥为种泥进行厌养氨氧化微生物的富集培养,在培养的第40d就具有了明显的厌氧氨氧化活性,在200d时活性达到2160mg·g-1·d-1(NH4+-N,以VSS计).采用分子生物学手段对培养得到的微生物进行鉴定,确定其为一种尚未被报道的可进行厌氧氨氧化反应的细菌.实验证明培养得到的厌氧氨氧化微生物是以NO2--N为电子受体进行反应,NO3--N不能直接被该厌氧氨氧化微生物利用;反应的适宜pH范围为7～8左右;无机碳源对其厌氧氨氧化活性有很大的影响,在缺少无机碳源时活性几乎被完全抑制.
- Abstract：Using nitration sludge as an inoculum, Anammox microorganisms were cultured in continuous stirred reactors. The obvious Anammox activity in the reactor was detected even after 40 days' cultivation. The activity in 200th day was 2160 mg·g-1·d-1 (NH4+-N,VSS). A novel type of bacterium responsible for Anammox process was identified through molecular biological methods. Experiments showed that nitrite rather than nitrate was used as electron acceptor through the Anammox process, and the optimal pH for the reaction was 7～8. In addition, inorganic carbon source was indispensable for the anammox process.
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