污染控制技术及原理
赵现方,王振河,武忠伟,陈林海,张凤秋.紫外辐照对活性污泥中菌群存活率的影响[J].环境科学学报,2007,27(7):1163-1167
紫外辐照对活性污泥中菌群存活率的影响
- Microbial survival rates in UV-irradiated activated sludge
- 赵现方
- 河南科技学院生命科技学院, 新乡 453003
- 王振河
- 河南科技学院生命科技学院, 新乡 453003
- 武忠伟
- 河南科技学院生命科技学院, 新乡 453003
- 陈林海
- 郑州大学离子束生物工程实验室, 郑州 450052
- 张凤秋
- 郑州大学离子束生物工程实验室, 郑州 450052
- 摘要:利用荧光原位杂交技术(Fluorescentin-situhybridization,FISH)特异性地标记了活性污泥中α-Proteobacteria、β-Proteobacteria、γ-Proteobacteria和Comamonas sp.4大类群的微生物,借助流式细胞术(Flowcytometry,FCM)检测了这些类群的相对含量.结果表明,上述微生物的相对含量分别为15.09%、20.50%、12.00%和8.37%.紫外线辐照3min后,活性污泥中菌群的相对含量分别变为20.75%、9.10%、81·89%和41·16%,这说明不同菌群对紫外线的敏感度不同.
- Abstract:Bacteria from the α , β and γ subclasses of Proteobacteria and Comamonas sp. in activated sludge were specifically labeled by fluorescent in-situ hybridization (FISH) and flow cytometry (FCM) was used to detect the relative amounts of these four microbial communities. The initial relative quantities were 15.09 %, 20.5 %, 12.00% and 8.37%. After exposure to ultraviolet radiation (UV) for 3 minutes, the relative amounts changed to 20.75 %,9.10 %,81.89 % and 41.16 % indicating that the four microbial communities present different sensitivities to UVlight.
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