环境毒理
解静芳,孟紫强.二氧化硫衍生物对小鼠心、肝、肺蛋白质的氧化损伤作用[J].环境科学学报,2006,26(7):1175-1179
二氧化硫衍生物对小鼠心、肝、肺蛋白质的氧化损伤作用
- Oxidative damage of sulfur dioxide derivatives on hearts, livers and lungs of mice
- 基金项目：国家自然科学基金资助项目(No.30230310;No.20477023);山西省自然科学基金资助项目(No.20031092;No.20021014)
- 作者
- 单位
- 解静芳
- 山西大学环境医学与毒理学研究所, 太原 030006
- 孟紫强
- 山西大学环境医学与毒理学研究所, 太原 030006
- 摘要：研究了二氧化硫(SO2)衍生物——亚硫酸钠和亚硫酸氢钠混合物(分子比为3:1),对小鼠心、肝、肺中蛋白质的氧化损伤作用,探讨其分子作用机制.连续5d对动物腹腔注射SO2衍生物,每天注射剂量为0、0.025、0.100、0.400g·kg-1.用2,4-二硝基苯肼比色法测定不同器官组织中蛋白质羰基含量.结果表明,SO2衍生物染毒剂量为0.025、0.100和0.400g·kg-1时,小鼠心、肝、肺蛋白质羰基含量染毒组与对照组相比均呈现不同程度的增加,不同器官羰基含量增量大小的次序为:肝>肺>心;其染毒剂量与心、肝、肺蛋白质羰基含量之间存在明显的剂量效应关系(p<0.05),线性拟合确定系数分别为0.894、0.893和0.903.由此认为,SO2衍生物可引起小鼠心、肝、肺组织蛋白质的氧化损伤,以及不同组织器官的蛋白质氧化损伤程度不同.
- Abstract：To study the protein oxidative damage and its molecular mechanism induced by sulfur dioxide (SO2) derivatives including sodium sulfite and sodium bisulfite mixture (3:1, mol)in hearts, livers and lungs of mice. The mice had been treated with abdominal cavity injection of sulfur dioxide derivatives at different concentrations (0、0.025、0.100、0.400 g·kg-1) once per day for 5 days. The protein carbonyl content was quantitated by using spectro photometry with 2,4-dinitrophenyl-hydrazine (DNPH). It was shown that SO2 derivatives might increase the carbonyl content of proteins from livers,lungs and hearts of mice in a dose-dependent manner. The order of increasing in carbonyl content of proteins was liver> lung> heart. The correlation coefficient was respectively 0.893,0.903 and 0.894(P=0.05). There are significantly the dose-effect relation between the dose of the chemicals and protein carbonyl content. These results lead to a conclusion that sulfur dioxide derivatives could cause oxidation damage of proteins from hearts, livers and lungs of mice. The extent of protein oxidative damage is different in different organs.
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