污染控制技术及原理
刘海燕,宦海琳,汪育文,李建宏,周耀明.微囊藻毒素降解菌S3的分子鉴定及其降解毒素的研究[J].环境科学学报,2007,27(7):1145-1150
微囊藻毒素降解菌S3的分子鉴定及其降解毒素的研究
- Molecular identification of a microcystin-degrading bacteria strain S3 and its biodegradation of microcystin
- 基金项目：人事部留学回国人员择优资助项目(2005)
- 作者
- 单位
- 刘海燕
- 南京师范大学生命科学学院, 南京 210097
- 宦海琳
- 南京师范大学生命科学学院, 南京 210097
- 汪育文
- 南京师范大学生命科学学院, 南京 210097
- 李建宏
- 南京师范大学生命科学学院, 南京 210097
- 周耀明
- 南京师范大学化学科学学院, 南京 210097
- 摘要：对一株具有强降解微囊藻毒素MC-LR能力的细菌S3进行了分子鉴定.测得该菌16SrDNA为1396bp,GenBank序列登录号为DQ836314.序列比对结果显示,该菌与类芽孢杆菌Paenibacillus validus的相似性达98%.微囊藻毒素降解实验结果表明,该菌能在以微囊藻毒素为唯一碳、氮源的培养基中生长,微囊藻毒素在72h内减少78.3%,菌株S3的最适生长温度是30℃,最适生长pH值为7.0.
- Abstract：More than 18 microcystin-degrading bacterial strains were isolated from water bodies with cyanobacteria blooms. The strain with the highest degrading capacity was strain S3. To identify the species, 16SrDNA of S3 was amplified by PCR. The PCRproduct was sequenced and the data submitted to GenBank under accession number DQ836314. BLASTsequence analysis showed that it had a 98% similarity to Paenibacillus validus. S3 could grow on mineral medium supplied with microcystins (mainly MC-LR), extracted from Microcystis PCC7806, as sole carbon and nitrogen sources (microcystins were substituted for NH4+ and glucose in M9 medium). S3 could degrade and metabolize 78.3% of 5 mg·L-1 MC-LRin 72h. To find suitable conditions for the bacterial degradation of MC-LR, we investigated the effects of temperature and pHon cell growth and MC-LRdegradation rate. The results showed that 30 ℃ and pH7.0 were the best conditions, which are similar to the environmental conditions for Microcystis blooms. So S3 may be useful in removing MCs from water during algal blooms.
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