• 关键词：氧化硫硫杆菌  温度  pH  重金属  耐受性

• 基金项目：国家自然科学基金项目(No.20377023);新世纪优秀人才计划(No.NECT-04-0505);江苏省自然科学基金重点项目(No.BK2004213)

• 黄峰源
• 南京农业大学资源环境学院环境工程系, 南京 210095

• 王世梅
• 南京农业大学资源环境学院环境工程系, 南京 210095

• 周立祥
• 南京农业大学资源环境学院环境工程系, 南京 210095

• 摘要：采用纯培养的方法研究了温度和介质起始pH以及重金属对嗜酸性氧化硫硫杆菌(Acidithiobacillus thiooxidansTS6)菌株生长与活性的影响.研究表明,菌株TS6的最适生长温度为28℃,最适起始生长pH范围为2.0～6.0.在上述条件下,TS6旺盛生长,表现出氧化硫能力最强.通过检测TS6对几种重金属(Cr³⁺、Cu²⁺、Zn²⁺、Ni²⁺)的耐受性,结果发现TS6对上述重金属均有较强的耐受性,至少可耐受Cr³⁺、Cu²⁺、Zn²⁺、Ni²⁺的浓度分别达1500、400、1000和250mg·L^-1.在相同的浓度下,不同重金属对TS6生长产酸作用的影响由小到大依次为Cr³⁺、Zn²⁺、Cu²⁺、Ni²⁺.随着TS6菌株驯化时间的延长,它们对重金属的耐受性可望得到进一步的增强,完全满足对各种污水污泥进行生物淋滤处理的条件.

• Abstract：Acidithiobacillus thiooxidans TS6 isolated from sewage sludge was studied for its optimum growth conditions and resistance to Cr³⁺, Cu²⁺, Zn²⁺, and Ni²⁺ by pure culture method. It was found that optimum temperature and initial pH in medium for strain TS6 growth were 28℃ and pH 2.0～6.0, respectively, as exhibiting that pH declined rapidly under these conditions. The sulfur-oxidizng activity of Acidithiobacillus thiooxidans TS6 was reduced obviously when cultural temperature was above 36℃ or below 24℃ and also initial pH in medium was more than pH 7. Furthermore, a higher resistance of Acidithiobacillus thiooxidans TS6 to Cr³⁺, Cu²⁺, Zn²⁺, and Ni²⁺ in the medium was also observed in the study. Acidithiobacillus thiooxidans TS6 could resist the attack of at least 1500 mg·L^-1 of Cr³⁺,400 mg·L^-1 of Cu²⁺, 1000 mg·L^-1 of Zn²⁺, and 250 mg·L^-1 of Ni²⁺ in medium. For the same metal concentration, the stress of different metal to strain TS6 was Ni²⁺ <Cu²⁺ < Zn²⁺ <Cr³⁺. In term of metal concentration in most of sewage sludge, strain TS6 could be used for bioleaching of heavy metals of sewage sludge.

• Key words：Acidithiobacillus thiooxidans  temperature  pH  heavy metal  resistance

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