生态毒理
聂湘平,王翔,陈菊芳,鹿金雁,李潇,杨永涛.三氯异氰尿酸与盐酸环丙沙星对蛋白核小球藻的毒性效应[J].环境科学学报,2007,27(10):1694-1701
三氯异氰尿酸与盐酸环丙沙星对蛋白核小球藻的毒性效应
- Toxic effects of trichloroisocyanuric acid and ciprofloxacin hydrochloride on a freshwater alga,chlorella pyrenoidosa
- 基金项目：国家自然基金项目(No40471118);国家自然科学基金委与广东省联合基金(NoU0633006)
- 作者
- 单位
- 聂湘平
- 暨南大学生命科学技术学院水生生物学国家重点学科 广州 510632
- 王翔
- 暨南大学生命科学技术学院水生生物学国家重点学科 广州 510632
- 陈菊芳
- 暨南大学生命科学技术学院水生生物学国家重点学科 广州 510632
- 鹿金雁
- 暨南大学生命科学技术学院水生生物学国家重点学科 广州 510632
- 李潇
- 暨南大学生命科学技术学院水生生物学国家重点学科 广州 510632
- 杨永涛
- 暨南大学生命科学技术学院水生生物学国家重点学科 广州 510632
- 摘要：测试了典型渔药三氯异氰尿酸(Trichloroisocyanuricacid,TCCA)、盐酸环丙沙星(Ciprofloxacinhydrochloride,CPFX)对蛋白核小球藻(Chlorellapyrenoidosa)的急性毒性及其Ⅰ相、Ⅱ相代谢酶活性的影响.结果表明,TCCA和CPFX作用小球藻96h后EC50分别为0.31mg·L-1和20.61mg·L-1.TCCA在低浓度下对小球藻Ⅰ、Ⅱ相代谢酶GSH、GST、CAT和EROD都存在诱导作用,当TCCA浓度大于0.13mg·L-1时,对GSH、GST和EROD的诱导减弱.GSH、GST、EROD三种酶对CPFX作用的响应较弱,当CPFX大于70.20mg·L-1时,GST受到显著抑制,而EROD则明显升高,与对照组差异显著,GSH没有明显变化.CAT对CPFX作用的响应较敏感,表现为典型适应性诱导现象"钟形曲线",适合作为CPFX暴露的生物标记物.
- Abstract：Adverse effects of trichloroisocyanuric acid(TCCA) and ciprofloxacin hydrochloride(CPFX) to a freshwater alga, Chlorella pyrenoidosa were assessed through toxicity bioassays based on cell survival and enzymatic activities in Phase Iand Phase II(GSH,GST and EROD).The results showed that 96h median effective concentrations(EC50) of TCCA and CPFX were 0.31 mg·L-1 and 20.61 mg·L-1,respectively.Enzymatic activities(GSH,GST and EROD) were found to be induced at low concentrations and decreased at higher concentrations of TCCA (>0.13 mg·L-1).No significant effects were observed for GSH,GST and EROD activities of Chlorella pyrenoidosa under low concentration exposure to CPFX,but when CPFX was above 70.20 mg·L-1,GST enzymatic activity decreased while EROD activity increased.CAT displayed a sensitive response to exposure to CPFX and exhibited a typical bell-shaped curve.Thus,CAT may be useful as a sensitive biomarker of exposure to CPFX.
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