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邓雅斌,杨玉荣.利用秀丽小杆线虫Caenorhabditis elegans监测厦门岛海水水质[J].环境科学学报,2007,27(6):1034-1037
利用秀丽小杆线虫Caenorhabditis elegans监测厦门岛海水水质
- Use of Caenorhabditis elegans as a pollution biosensor in Xiamen Seawater
- 基金项目：国家自然科学基金项目(No.30370695)
- 作者
- 单位
- 邓雅斌
- 厦门大学生命科学学院, 厦门 361005
- 杨玉荣
- 厦门大学生命科学学院, 厦门 361005
- 摘要：采用野生型秀丽小杆线虫与带有hsp16启动子的转基因虫株分别对厦门岛附近5个站位的表层水样污染程度进行了监测,并将致死率和染色率的检测结果进行比较.结果表明,秀丽小杆线虫的致死率和染色率具有相关性.应用模式生物秀丽小杆线虫对环境及污染物的监测的方法可行,用转基因的秀丽小杆线虫能够提高检测的灵敏度,缩短检测时间.
- Abstract：Caenorhabditis elegans was applied as a pollution biosensor because of its small size, high fecundity and short generation time. Its mortality is a biomonitoring criterion which indicates the degree of pollution. In this study, both the mortality of the wild-type worm and the staining of a transgenic strain were used to test the quality of surface water samples from five stations in the Xiamen sea area. Comparison of both results show that mortality and staining were correlated. Using C.elegans stress-inducible transgenic worms as biosensors could shorten the testing time and improve the sensitivity of environmental monitoring.
- Key words：nematode sea pollution mortality HSP transgenic
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