污染控制技术及原理
任南琪,许丽英,张颖,徐慧,王兴祖,陈冠雄.产氢发酵新菌Ethanoligenens sp.B49对铁的依赖性及产氢途径初探[J].环境科学学报,2006,26(10):1643-1650
产氢发酵新菌Ethanoligenens sp.B49对铁的依赖性及产氢途径初探
- Dependence on iron and hydrogen producing pathway for novel strain Ethanoligenens sp.B49
- 基金项目：国家自然科学基金(No.30470054)
- 作者
- 单位
- 任南琪
- 哈尔滨工业大学环境生物技术研究中心, 哈尔滨 150090
- 许丽英
- 哈尔滨工业大学环境生物技术研究中心, 哈尔滨 150090
- 张颖
- 中国科学院应用生态研究所, 沈阳 110016
- 徐慧
- 中国科学院应用生态研究所, 沈阳 110016
- 王兴祖
- 哈尔滨工业大学环境生物技术研究中心, 哈尔滨 150090
- 陈冠雄
- 中国科学院应用生态研究所, 沈阳 110016
- 摘要：采用典型产氢途径的丁酸梭菌和阴沟肠杆菌作为对比菌株,铁元素作为限制性因子,研究Ethanoligenens sp.B49的产氢代谢过程对铁的依赖性以及产氢机制.铁限制性培养结果表明,与完全培养条件相比,在铁浓度限制在2mg·L-1条件下B49仍保持很好的生长状态和产氢性能,其产氢不受铁元素不足的影响,即其产氢行为不具有铁依赖性;这一性质与梭菌型产氢途径的丁酸梭菌表现出相似的规律,完全区别于阴沟肠杆菌铁依赖型的产氢机制.初步判断高效产氢菌B49的产氢代谢途径类似于梭菌型产氢途径.
- Abstract：The hydrogen-producing pathway of Ethanoligenens sp. B49 was investigated. As a metabolic limiting factor, the influence of iron on the hydrogen production in the batch culture fermentation process was evaluated. Besides, Clostridium butyricum and Enterobacter cloaca which have typesof typical hydrogen-producing pathways were adopted for comparison with strain B49. The results showed that the activity and hydrogen-producing capacity of strain B49 varied little under the conditions of adding and without adding iron, which implied that hydrogen-producing behavior of Ethanoligenens sp. B49 did not depend on iron. This characteristic was similar to that of Clostridium butyricum but quite different from Enterobacter cloaca with Coli-type hydrogen-producing pathway. Therefore, the hydrogen production by Ethanoligenens sp. B49 was probably by Clostridial type pathway.
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