• 关键词：异丙甲草胺  蚯蚓  体重  酶活性

• 基金项目：国家自然科学基金资助项目(No20677049)

• 刘文丽
• 浙江大学环境科学研究所, 杭州 310029

• 徐冬梅
• 浙江大学环境科学研究所, 杭州 310029

• 刘惠君
• 浙江大学环境科学研究所, 杭州 310029

• 刘广深
• 浙江大学环境科学研究所, 杭州 310029

• 摘要：采用自然土壤法,研究了异丙甲草胺对蚯蚓体重及体内纤维素酶、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性的影响.结果表明,蚯蚓体重及纤维素酶活性在异丙甲草胺作用下均受到抑制;蚯蚓体内SOD、CAT、POD活性对异丙甲草胺响应不同,CAT活性受影响最大,POD次之,SOD最小.异丙甲草胺浓度对蚯蚓体重及体内纤维素酶和CAT活性影响显著,对SOD和POD则无显著性影响;处理时间对蚯蚓体重及体内纤维素酶、SOD、CAT和POD活性均有极显著影响;而异丙甲草胺浓度和处理时间二者交互作用对蚯蚓体内CAT和POD活性有极显著影响,对蚯蚓体重及体内纤维素酶和SOD活性则影响不大.

• Abstract：The natural soil test method was used to study the effects of metolachlor on the weight and enzyme activities of earthworms(Eisenia fetida).The activities of cellulase,superoxide dismutase(SOD),catalase(CAT) and peroxidase(POD) were measured.The weight and cellulase activity of earthworms were inhibited after exposure to metolachlor.The activities of SOD,CATand PODof the earthworm showed different responses to metolachlor,in the sensitivity order CAT>POD>SOD.The weight,cellulase activity and CATactivity were significantly affected by metolachlor concentration,and all parameters were significantly affected by the duration of exposure.The interaction exposure concentration and duration had significant effects on the activities of CATand POD,but had little effect on the weight,the activities of cellulase and SOD.

• Key words：metolachlor  earthworm  weight  enzyme activities

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