• 关键词：六价铬  DNA损伤  肝脏  肾脏  氧化应激

• 基金项目：国家自然科学基金重点项目(No.20137010)

• 王晓峰
• 浙江大学医学院生物化学与遗传学系, 杭州 310058

• 楼建林
• 浙江大学劳动卫生与环境卫生研究所, 杭州 310058

• 邢鸣鸾
• 浙江大学医学院生物化学与遗传学系, 杭州 310058

• 连灵君
• 浙江大学医学院生物化学与遗传学系, 杭州 310058

• 徐立红
• 浙江大学医学院生物化学与遗传学系, 杭州 310058

• 摘要：为研究六价铬(Cr(Ⅵ))摄入后对机体造成的损伤,将重铬酸钾(K₂Cr₂O₇)以25、50、100mg·kg^-1对小鼠进行灌胃,染毒时间为1d、3d和5d.检测外周血淋巴细胞DNA损伤及肝肾组织活性氧自由基(ROS)水平、脂质过氧化产物丙二醛(MDA)含量,以及超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性.实验结果表明,经Cr(Ⅵ)染毒1d、3d和5d后小鼠外周血淋巴细胞DNA损伤及肝ROS水平与对照组相比均有明显升高,肝脏抗氧化酶活性也有显著改变,但MDA含量差异不显著,而肾组织中各指标均未见有明显改变.由此认为,经口摄入Cr(Ⅵ)后能导致DNA损伤并且对肝脏造成氧化应激.

• Abstract：To investigate the toxic effects of hexavalent chromium(Cr(Ⅵ)), mice were gavaged with potassium dichromate at the dose of 25, 50, 100. mg·kg^-1 for 1 day, 3 days and 5 days. The DNA damage of peripheral blood lymphocytes, reactive oxygen species level (ROS), malondialdehyde (MDA) content and the activities of superoxide dismutase (SOD) and catalase (CAT) in liver and kidney were measured. The results showed that enhanced production of DNA damage in peripheral blood lymphocytes and significant changes in ROS level and SOD, CAT activities in liver were observed by the administration of Cr(Ⅵ) as compared to the control animals, but no prominent alterations were shown in the MDA content. There were no significant differences on the four parameters in the kidney between the control and treatment groups. These results indicate that oral administration of Cr(Ⅵ) can induce DNA damage and hepatic oxidative stress

• Key words：hexavalent chromium  DNA damage  liver  kidney  oxidative stress

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