• 关键词：旅游活动  张家界国家森林公园  微生物生物量碳、氮、磷

• 基金项目：湖南省自然科学基金(No.05JJ30152);湖南农业大学人才基金项目(No.05WD01)

• 谭周进
• 湖南农业大学资源与环境微生物研究所, 长沙 410128

• 戴素明
• 湖南农业大学资源与环境微生物研究所, 长沙 410128

• 谢桂先
• 湖南农业大学资源与环境微生物研究所, 长沙 410128

• 田慧
• 湖南农业大学资源与环境微生物研究所, 长沙 410128

• 摘要：为了探明旅游活动对张家界国家森林公园土壤微生物活性及多样性的影响,保护土壤生态平衡及合理开发自然保护区提供理论依据,进行了旅游踩踏对土壤微生物生物量碳、氮、磷的影响研究.结果表明,在所设的3个试验区中,0～5cm土壤层的微生物生物量碳、磷受到旅游踩踏的影响最严重,5～15cm土壤层的微生物生物量碳、磷受到旅游踩踏的影响较严重,而15～25cm土壤层的微生物生物量碳、磷受到旅游踩踏的影响最轻,旅游踩踏对所设3个试验区中3个层次土壤的微生物生物量碳、磷的影响均达到了显著水平(p<0.05).从旅游踩踏对3个土壤层的微生物生物量氮的影响程度来看,0～5cm土壤层的微生物生物量氮受到的影响最严重,5～15cm土壤层的微生物生物量氮受到的影响较严重,而15～25cm土壤层的微生物生物量氮受到的影响最轻,背景区与缓冲区15～25cm土壤层的微生物生物量氮差异没有达到显著水平.说明张家界国家森林公园土壤微生物遭到了旅游踩踏的破坏,抵御外界干扰的能力已受到了旅游踩踏的破坏.

• Abstract：Ecological environment was destroyed by recreation activities. In order to probe into the effect of recreation activities on microbes in soil and to provide useful development suggestion, the studies on the effect of recreation on microbial biomass carbon, microbial biomass nitrogen and microbial biomass phosphorus in soil from Zhangjiajie National Forest Park were conducted. The results showed that: When soils from control zero, buffer zero and active zero were used for analysis, the microbial biomass carbon and phosphorus from 0～5 cm layer soil was affected by human's recreational activities seriously. And the microbial biomass carbon and phosphorus from 5～15 cm layer soil and from 15～25cm layer soil was affected prominently by human kind's recreation activities.The microbial biomass carbon and phosphorus from 0～5cm layer soil, 5～15cm layer soil and 15～25cm layer soil in control zero, buffer zero and active were affected prominently by recreation activities(P<0.05). The microbial biomass nitrogen from 0～5cm layer soil, 5～15cm layer soil and 15～25cm layer soil in control zero, buffer zero and active were affected by recreation activities. Except the difference between the 15～25 cm soil layer from control zero and buffer zero, the difference of microbial biomass nitrogen from the other soils were prominent(P<0.05).All these results showed that the biodiversity and defense ability of ecosystem in Zhangjiajie National Forest Park was destroyed, and it's necessary to make measures to protect Zhangjiajie National Forest Park from being destroyed.

• Key words：recreation activities  Zhangjiajie National Forest Park  microbial biomass carbon, nitrogen and phosphorus

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