• 关键词：NMDA-R  β-actin  气态甲醛  中枢神经毒性  学习和记忆  RT-PCR

• 基金项目：国家自然基金面上项目(No.30570799);湖北省科技攻关计划项目(No.2005AA307B01)

• 柯珂
• 华中师范大学生命科学学院环境科学实验室, 武汉 430079

• 朱燕
• 华中师范大学生命科学学院环境科学实验室, 武汉 430079

• 乔琰
• 华中师范大学生命科学学院环境科学实验室, 武汉 430079

• 彭光银
• 华中师范大学生命科学学院环境科学实验室, 武汉 430079

• 徐钱
• 华中师范大学生命科学学院环境科学实验室, 武汉 430079

• 杨旭
• 华中师范大学生命科学学院环境科学实验室, 武汉 430079

• 摘要：通过测定小鼠前脑N—甲基—D-天冬氨酸受体(N-methyl-D-aspartatereceptor,NMDA-R)的转录水平的变化,研究中低浓度甲醛暴露环境对小鼠中枢神经系统的影响.实验以昆明系小鼠为实验材料,经甲醛染毒后,立即脱颈处死并取其前脑组织提取总RNA,采用RT-PCR二步法对其中的NMDA-R亚基(Grin1,Grin2a,Grin2b)及管家基因β-actin进行扩增,通过光密度分析染毒前后基因转录水平的变化.实验结果表明,与对照组相比,0.5mg·m^-3浓度的气态甲醛可使Grin1基因转录稍有上调,但无显著性差异;Grin2a基因转录上调且有显著性差异(p<0.05),Grin2b基因略有下调但无显著性差异.3.0mg·m^-3浓度的甲醛可使Grin1基因转录上调并具有极显著差异(p<0.01),且上调幅度明显大于0.5mg·m^-3染毒组;Grin2a基因转录显著性上调(p<0.05),但上调幅度小于0.5mg·m^-3染毒组;Grin2b基因转录显著性下调(p<0.05).由此可见,低浓度甲醛(0.5mg·m^-3)的吸入对NMDA-R(除Grin2a外)的各亚基的影响较小.中等浓度甲醛(3.0mg·m^-3)的吸入对NMDA-R影响较大,这可能是持续性气态甲醛吸入对小鼠中枢神经系统产生影响的重要机制之一.

• Abstract：The effects of middle and low concentrations of inhaled formaldehyde exposure on central nervous system were studied in this research by examining the transcriptional changes of N-methyl-D-aspartate receptor (NMDA-R) in mice fore cerebrum. Kunming purebred mice were used as experimental materials, and the total RNAwas extracted from the cerebrum immediately after exposed to formaldehyde. RT-PCRwas applied to examine the transcriptional changes of NMDA-Rsubunits (Grin1, Grin2a, Grin2b) and β-actin which was used as control gene. The results showed that 0.5 mg·m^-3 formaldehyde exposure up-regulated Grin1 slightly but without significance, up-regulated Grin2a significantly (compared with control groups, p<0.05), and down-regulated Grin2b slightly. 3.0mg·m^-3 formaldehyde exposure up-regulated Grin1 significantly (p<0.01) as well as Grin2a (p<0.05), and down-regulated Grin2b significantly (p<0.05). These results indicated that low concentrations of inhaled formaldehyde have little effect on the transcription of NMDARsubunits except Grin2a. While middle concentrations of inhaled formaldehyde could influence the transcription of NMDARsubunits significantly, such influence may be one of the important mechanisms of effects of continuous gaseous formaldehyde on central nervous system.

• Key words：NMDA-R  β-actin  gaseous formaldehyde  central nervous system toxicity  learning ability and memory  RT-PCR

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