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Improved remediation of waste water by Hydrilla verticillata grown with chitosan

摘要：利用化学需氧量(COD_Cr)分别为318mg·L^-1、157mg·L^-1、30mg·L^-1的生活污水培养轮叶黑藻.在各浓度生活污水中添加浓度为0.1%的壳聚糖,同时设不添加壳聚糖的对照组.通过对轮叶黑藻生长指标和叶绿素、可溶性蛋白质含量及抗氧化酶活性的测定,研究壳聚糖对沉水植物轮叶黑藻抗污能力的影响.结果显示,壳聚糖可明显促进轮叶黑藻主茎、根、分枝的生长,添加壳聚糖的试验组植株的叶绿素、可溶性蛋白质的含量比对照组高,且差异显著.壳聚糖能诱导黑藻超氧化物歧化酶和过氧化物酶活性增高,减轻膜脂质过氧化反应.研究结果提示壳聚糖能够促进黑藻生长,提高黑藻的抗污能力.

Abstract：The effects of chitosan on phytoremediation of wastewater by Hydrilla verticillata were studied by cultivating Hydrilla verticillata in sewage with different COD_Cr concentrations(318、157 and 30 mg·L^-1).At each concentration,0.1% chitosan was added to the experimental group while the other,with no chitosan added,served as a control.The growth,the content of chlorophyll,dissolved protein and activity of antioxidant enzymes inside the body of Hydrilla verticillata were measured.The results indicated that the chitosan made the growth of main caulis,roots and ramifications much faster.The content of chlorophyll and dissolved protein of the experiment group were higher than that of the control.Chitosan can induce the SODand PODactivities in the plant,weaken lipid peroxidation reactions and improve the ability of Hydrilla verticillata to remediate waste water.