• 关键词：膜生物反应器  膜污染  胞外聚合物  电泳

• 基金项目：天津市科技发展计划项目(No.043115111-5)

• 张斌
• 1. 天津大学环境科学与工程学院, 天津 300072; 2. 军事医学科学院卫生学环境医学研究所, 天津 300050

• 孙宝盛
• 天津大学环境科学与工程学院, 天津 300072

• 金敏
• 1. 天津大学环境科学与工程学院, 天津 300072

• 龚泰石
• 军事医学科学院卫生学环境医学研究所, 天津 300050

• 摘要：研究了胞外聚合物对膜生物反应器中膜污染物的贡献和影响,通过对膜表面的EDX分析,发现膜污染物以有机污染物为主;采用碱液离心提取法提取反应器污泥和膜污染物中的EPS,发现二者的EPS主要由相对分子量在12000以上的大分子物质组成,而且这些大分子物质基本上都是难降解的;以TOC表征EPS总量,并对其中多糖、蛋白质、DNA等组分进行测定,发现膜污染物中的EPS浓度是反应器污泥中的4倍左右,多糖类物质是首要的膜污染物,蛋白质对膜污染也起着重要的作用;反应器内DNA含量要高于膜污染物中,可证实DNA不是主要的膜污染物;通过电泳分析发现,二者大片段区间内的DNA的分子量几乎相同.

• Abstract：The effect of Extracellular Polymeric Substances (EPS) on membrane fouling was investigated. The new membrane and fouling membrane surface morphology were characterized by EDXanalysis respectively. The results show that membrane contaminant is mainly composed of organic compounds. EPSin active biomass and membrane pollutant were extracted by method of alkaline regular centrifugation. Most of EPSwere found to have high molecular weight over 12000D,which is very difficult to be degraded. The total EPScontents in the extracted solution were measured TOC. And the contents of polysaccharide、protein and DNAin EPSwere analyzed respectively. The analytical results indicate that the content of EPSin membrane fouling is about 4 times as much as that in active biomass. Polysaccharide is the most important pollutant on membrane, and also protein's role in membrane fouling is not negligible, whereas the DNAis not important in membrane fouling because of its content (non-dialysis) in membrane contaminant being less than that in the active biomass. In addition, the results in electrophoresis experiment provide that the concentrated distributions of molecular weight of large fragment DNAare similar in both membrane contaminant and the biomass.

• Key words：MBR  membrane fouling  EPS  electrophoresis

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