环境毒理
曹强,钱孝琳,张澍,宋伟民.大气细颗粒物水溶成分和非水溶成分的细胞毒性[J].环境科学学报,2008,28(6):1167-1172
大气细颗粒物水溶成分和非水溶成分的细胞毒性
- Cytotoxicity of soluble and insoluble components of atmospheric fine particles
- 基金项目：国家自然科学基金专项经费资助(No.30271106);国家自然科学基金重大项目(No.10490182)
- 作者
- 单位
- 曹强
- 复旦大学公共卫生学院环境卫生教研室, 上海 200032
- 钱孝琳
- 复旦大学公共卫生学院环境卫生教研室, 上海 200032
- 张澍
- 上海国际机场检验检疫局, 上海 201202
- 宋伟民
- 复旦大学公共卫生学院环境卫生教研室, 上海 200032
- 摘要：为了研究细颗粒物不同成分细胞毒作用的差异,以细颗粒物水溶成分和非水溶成分对人肺癌上皮细胞株A549细胞染毒.结果表明,在染毒剂量为100μg·mL-1、200μg·mL-1和300μg·mL-1的范围内,水溶成分的细胞毒性明显高于非水溶成分(P<0.05),表现为抑制细胞活性,导致细胞膜损伤增加,并且诱导细胞活性氧(ROS)的产生.使用N-乙酰半胱氨酸(NAC)和抗坏血酸(VitC)阻断活性氧(ROS)产生的研究结果表明,与未加上述抗氧化剂的染毒组相比,加抗氧化剂的染毒组细胞的活性明显增强,因此,提示细颗粒物水溶成分是最主要的毒性成分之一,而氧化-应激损伤可能是细颗粒物细胞毒作用的重要机制之一.
- Abstract：In order to study the cytotoxicity of different components of fine particles,cells of the human lung adenocarcinoma cell line(A549 cells)was exposed to soluble and insoluble components of fine particles collected from ambient air in urban district of Shanghai.The results showed that soluble components were more toxic than insoluble components to cells exposed to 100 μg·mL-1,200 μg·mL-1 and 300 μg·mL-1 of each type(P<0.05).Compared with insoluble components,soluble components showed more inhibition of cell activity,greater injury of cell membranes,and larger quantities of reactive oxygen species(ROS).Treatment of the cells with soluble components(300 μg·mL-1)in the presence of the antioxidants N-Acetyl-L-cysteine(NAC) or Vitamin Cshowed that ROSlevels decreased greatly,while cell activity increased significantly compared with cells treated in the absence of the antioxidants.However,the antioxidants offered little protection against the effects of the insoluble components.This result suggests that the insoluble components are one of the most cytotoxic components of fine particles,and that oxidative stress is one of the mechanisms of lung injury caused by fine particles.
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