污染控制技术及原理
杨基先,高珊珊,马放,苏俊峰,王强.一株好氧反硝化细菌的分离鉴定及反硝化能力[J].环境科学学报,2008,28(7):1302-1307
一株好氧反硝化细菌的分离鉴定及反硝化能力
- Identification and phylogenetic analysis of an isolated aerobic denitrifier
- 基金项目：国家自然科学基金(No.50778052);黑龙江省杰出青年科学基金(No.JC200601)
- 作者
- 单位
- 杨基先
- 哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090
- 高珊珊
- 哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090
- 马放
- 哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090
- 苏俊峰
- 哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090
- 王强
- 哈尔滨工业大学城市水资源与水环境国家重点实验室, 哈尔滨 150090
- 摘要：利用驯化活性污泥的方法筛选出一株好氧反硝化细菌G3,该菌在碳源充足且溶解氧为(4.0±1.0)mg·L-1条件下,24h内对NO2--N积累量为12.03mg·L-1,对NO3--N的去除率为91.09%.扫描电镜观察其形态特征为(0.4～0.6)μm×(1.2～2.0)μm的短杆菌.16SrDNA序列分析表明,该菌与Pseudomonas stutzeri的相似性为100%.利用MEGA软件对该菌株与已报道的相关微生物进行系统发育分析,并克隆出与好氧反硝化相关的周质硝酸盐还原酶的亚基napA基因,初步判定筛选菌株具有周质硝酸盐还原酶.
- Abstract：By domesticating activated sludge in a SBRreactor with alternation of anaerobic/aerobic processes, the bacterium Pseudomonas stutzeri strain G3 was isolated, which is capable of aerobic denitrification with sodium succinite as sole carbon source. When the concentration of dissolved oxygen was(4.0±1.0)mg·L-1, the nitrite accumulation and the removal rate of nitrate by strain G3 were 12.03 mg·L-1 and 91.09% in24 hours, respectively; when C/N=3 the removal rate of nitrate by the bacterium cultured at 30℃ for 28 h was 72.91%. The morphology of the bacterium was a brevibacillus with average size of (0.4～0.6)μm×(1.2～2.0)μm as measured by scanning electron microscopy. Phylogenetic analysis based on partial 16SrDNAand performed by MEGAshowed that G3 has 100% sequence similarity with Pseudomonas stutzeri. The phylogenetic tree of the strain and its neighboring denitrifiers is given. Its partial napAgene was cloned, and shown to be a subunit of periplasmic nitrate reductase(NAR), which is considered to be related to aerobic denitrification.
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