• 关键词：尼古丁  Pseudomonas sp.  生物降解

• 基金项目：浙江省科技厅重点项目(No.2007C23035)

• 孙柯丹
• 浙江工业大学生物与环境工程学院, 杭州 310032

• 朱晨静
• 浙江工业大学生物与环境工程学院, 杭州 310032

• 钟卫鸿
• 浙江工业大学生物与环境工程学院, 杭州 310032

• 陈建孟
• 浙江工业大学生物与环境工程学院, 杭州 310032

• 叶紫娟
• 杭州利群环保纸业有限公司, 杭州 310018

• 刘培基
• 杭州利群环保纸业有限公司, 杭州 310018

• 周强
• 杭州利群环保纸业有限公司, 杭州 310018

• 摘要：从杭州利群环保纸业有限公司库存废烟叶中分离得到一株尼古丁高效降解菌株ZUTSKD.经形态、生理生化以及16SrDNA序列同源性分析,鉴定该菌株属于Pseudomonas sp.,命名为Pseudomonas sp.Strain ZUTSKD.该菌能以尼古丁为唯一碳氮源和能源生长,降解尼古丁最适温度为30℃,最适pH为7.5.添加氯化铵和葡萄糖有利于尼古丁的降解.在尼古丁的代谢产物中能够检测到3-(2,3,4-三氢—5-吡咯基)—吡啶,2,3′-二吡啶,可天宁和3-羧基—吡啶.该研究表明,ZUTSKD菌株有很强的尼古丁降解能力,可用于处理烟草废弃物.

• Abstract：A novel nicotine-degrading bacterium isolate named ZUTSKDwas isolated from waste tobacco. Based on standard morphological and physiological properties and nucleotide sequence analysis of enzymatically amplified 16Sribosomal deoxyribonucleic acid, it was identified as Pseudomonas sp. Nicotine could be utilized as sole source of carbon, nitrogen and energy by ZUTSKD. The optimal conditions for nicotine degradation are at 30℃ with initial pH7.5 The addition of NH₄Cl and glucose could improve the nicotine degradation by ZUTSKD. Four novel intermediate metabolites, 3-(3, 4-dihydro-2H—pyrrol-5-yl)—pyridine, 2,3′-bipyridine, cotinine and 3-pyridinecarboxylic acid, were detected in the culture medium. ZUTSKDshowed high ability to degrade nicotine and is a potential strain for direct application in tobacco waste treatment.

• Key words：nicotine  Pseudomonas sp.  biodegradation

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