• 关键词：镉  鸡垂体  凋亡  Fas  caspase-3

• 基金项目：黑龙江省教育厅资助项目(No10551038)

• 何宝霞
• 1. 东北农业大学动物医学院, 哈尔滨 150030; 2. 周口职业技术学院动物科学系, 周口 466001

• 傅业全
• 周口职业技术学院动物科学系, 周口 466001

• 李金龙
• 东北农业大学动物医学院, 哈尔滨 150030

• 徐世文
• 东北农业大学动物医学院, 哈尔滨 150030

• 摘要：探讨氯化镉(CdCl₂)诱导鸡脑垂体细胞凋亡的发生情况及对凋亡相关基因Fas和caspase-3表达的影响.选择健康50日龄海兰白蛋鸡90只,分为3组,每组30只,以CdCl₂含量为0、140、210mg·kg^-1的拌料饲喂,分别于染毒20d、40d和60d时取垂体,用原位末端标记(Tunel)和RT-PCR方法检测细胞凋亡和基因表达情况.实验结果表明,CdCl₂作用后可致鸡腺垂体细胞呈现明显的凋亡征象,并可诱导垂体细胞Fas和caspase-3mRNA表达的增加;在整个试验期的各个时间点2个剂量组的凋亡率和基因表达量与对照组相比差异显著(p<0.01),且随着染镉时间的延长,低剂量组的凋亡率以及Fas、caspase-3mRNA表达增加较明显.这表明,一定剂量的CdCl₂可诱导鸡垂体细胞凋亡,在此过程中Fas和caspase-3mRNA表达呈现出与凋亡较为一致的趋势.

• Abstract：The effect of cadmium chloride on cell apoptosis and the expressions of Fas and caspase-3 genes were explored in a hen model, Ninety normal Hyline white laying hens, aged 50 days, were randomly allocated to 3 groups of 30. The hens were fed 0 mg·kg^-1 CdCl₂ in the control group, 140 mg·kg^-1 CdCl₂ in the low-dosage cadmium group and 210 mg·kg^-1CdCl₂ in the high-dosage cadmium group. The anterior pituitary glands of the hens were removed after 20 d, 40 d and 60 d and the levels of cell apoptosis were detected by TUNEL and the expressions of Fas and caspase-3 genes were measured by RT-PCR. The results indicated that cadmium chloride can lead to apoptosis of pituicytes and overexpression of Fas and caspase-3 genes. At the same time point, cell apoptosis rates and gene expression showed variance (p<0.01) in both test groups compared with control and the low-dosage group changed greatly both the expression of Fas, caspase-3 mRNAand cell apoptosis rates. The data indicated that cadmium chloride could induce apoptosis of anterior pituitary cells. The expression of Fas and caspase-3 mRNAhave the same trend as apoptosis with the extension of time.

• Key words：cadmium  anterior pituitary of chicken  apoptosis  Fas  caspase-3

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