• 关键词：四溴双酚-A(TBBPA)  五溴酚(PBP)  甲状腺激素(THs)  脱碘酶(IDs)  红鲫

• 基金项目：国家自然科学基金(No20507007);长江水环境教育部重点实验室开放基金(NoYRWEF06003);上海市城市化过程与生态恢复重点实验室开放基金(NoUPER0606)

• 瞿璟琰
• 华东师范大学资源与环境学院环境科学系, 上海市城市化过程与生态恢复重点实验室, 上海 200062

• 施华宏
• 华东师范大学资源与环境学院环境科学系, 上海市城市化过程与生态恢复重点实验室, 上海 200062

• 刘青坡
• 华东师范大学资源与环境学院环境科学系, 上海市城市化过程与生态恢复重点实验室, 上海 200062

• 沈江帆
• 上海市瞢陀区中心医院核医学科, 上海 200062

• 摘要：将红鲫(Carassius auratus)分别暴露于0.5mg·L^-1四溴双酚-A(TBBPA)和0.1mg·L^-1五溴酚(PBP)中28d,采用放射性免疫分析法和化学发光酶联免疫分析法检测红鲫的血清甲状腺激素水平和肝脏脱碘酶活性.结果显示,TBBPA能使红鲫血清TT₄和TT₃水平降低,rT₃水平、肝脏脱碘酶ID2和ID3活性升高.PBP对红鲫甲状腺系统的干扰要小于TBBPA,它对红鲫血清TT₃水平和ID₂活性几乎没有影响.上述结果表明,TBBPA和PBP能够干扰红鲫体内甲状腺激素的稳态,加速甲状腺激素在肝脏内的转化和代谢;这可能是通过抑制甲状腺激素与运载蛋白的结合来实现的.

• Abstract：To test the thyroid disrupting effects of brominated flame ratardants in vivo,goldfish (Carassius auratus) were exposed to 0.5 mg·L^-1 tetrabromobisphenol-A (TBBPA) and 0.1 mg·L^-1 pentabromophenol (PBP) singly for 28 d. Plasma thyroid hormone levels and liver deiodinase activities were detected using microparticle enzyme immunoassay and radioimmunoassay. The results showed that TBBPA could decrease plasma TT₄ and TT₃ levels,and increase plasma rT₃ level and liver IDs activities. The disturbance of PBP was less than that of TBBPA. No effects were observed on the plasma TT₃ level or liver ID2 activity in groups exposed to PBP. It was deduced that TBBPA and PBP could interfere with the homeostasis of fish thyroid hormones,which might arise from interference with the binding of thyroid hormones to transthyretin.

• Key words：tetrabromobisphenol-A (TBBPA)  pentabromophenol (PBP)  thyroid hormones(THs)  deiodinases (IDs)  Carassius auratus

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