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环境毒理

杨维东,李丽璇,刘洁生,张剑军.海洋底栖甲藻——利玛原甲藻(Prorocentrum lima)对三种赤潮藻的化感作用[J].环境科学学报,2008,28(8):1631-1637

Allelopathy of marine benthic dinoflagellate-Prorocentrum lima on three red tide algae

基金项目：国家重点基础研究发展规划(973)项目(No2001CB409710,2001CB409709);广东省自然科学基金(No031885);国家自然科学基金-广东省联合基金重点项目(NoU0733006)

摘要：为明确利玛原甲藻(Prorocentrum lima)对其它生物可能存在的化感作用,考察了利玛原甲藻培养物、无藻细胞滤液以及腹泻性贝类毒素(diarrhetic shellfish poisoning,DSP)粗提物对塔玛亚历山大藻(Alexandrium tamarense)、海洋卡盾藻(Chattonella marina)和东海原甲藻(Prorocentrum donghaiense)3种赤潮藻生长的影响.结果显示,共培养时利玛原甲藻对其它3种赤潮藻的生长有不同程度的抑制作用,其生长也受到3种赤潮藻的影响;无藻细胞滤液对东海原甲藻和海洋卡盾藻有抑制作用,其中对东海原甲藻的抑制作用更明显,但对塔玛亚历山大藻的影响不大;比较而言,DSP粗提物对3种藻的影响最为明显,甚至可完全抑制海洋卡盾藻的生长.这些结果提示,利玛原甲藻与赤潮藻间存在交互抑制作用,可能会通过分泌化感物质、细胞间接触抑制等途径抑制其它藻的生长;利玛原甲藻具有一定的化感作用,但DSP毒素并非利玛原甲藻发挥化感效应的主要原因.

Abstract：In order to learn the potential allelopathic effect of Prorocentrum lima on other organisms,the influences of the fresh culture,cell-free filtrate and diarrhetic shellfish poisoning (DSP) toxin extract from P. lima on three microalgae,Alexandrium tamarense,Chattonella marina and Prorocentrum donghaiense,were observed. Growth of A. tamarense,C. marina and P. donghaiense were inhibited,when co-cultured with P. lima or cultured in f/2 medium with DSPtoxin. C. marina and P. donghaiense but not A. tamarense were heavily inhibited by the cell-free filtrate from P. lima. These results suggested that there was a reciprocal inhibition between P. lima and the three algae,and that P. lima could inhibit the three algae by allelochemicals and cell contact-inhibition. Other chemicals excreted from P. lima may be responsible for the allelopathic actions,but this needs further study.