• 关键词：铜绿微囊藻  氮限制  磷限制  生长  产毒

• 基金项目：国家重点基础研究发展计划项目(No.2007CB407301)

• 代瑞华
• 1. 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085; 2. 中国科学院研究生院, 北京 100039

• 刘会娟
• 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085

• 曲久辉
• 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085

• 赵旭
• 中国科学院生态环境研究中心环境水质学国家重点实验室, 北京 100085

• 摘要：采用分批培养的方式研究了铜绿微囊藻(Microcystis aeruginosa)在氮、磷单因子营养限制条件下的生长和产毒情况.结果表明,氮限制对铜绿微囊藻的生长和产毒都有明显的抑制作用,而磷限制对其生长和产毒没有明显的抑制作用.在氮限制条件下,铜绿微囊藻的生物量仅为控制组的30%～50%,产毒量也仅为控制组的10%～60%;并且氮限制会促进细胞分裂,缩短细胞的生长周期.这可能是因为氮是铜绿微囊藻生长和产毒所必需的元素,直接控制着铜绿微囊藻的生长和产毒;所以若培养基中的氮浓度比较低,会直接导致微囊藻的生物量较少和产毒量比较低.磷限制甚至磷被耗尽时对铜绿微囊藻的生长和产毒都影响不大,这可能与铜绿微囊藻体内储存的含磷颗粒物有关.实验结果表明,在本实验条件下,水体中氮限制对铜绿微囊藻的生长和产毒影响较大.

• Abstract：The effects of nitrogen-limitation and phosphorus-limitation on the growth and microcystin production of Microcystis aeruginosa were investigated in batch culture.The results demonstrated that the growth and microcystin production were largely retarded by nitrogen-limitation.But they were not influenced by phosphorus-limitation.Compared with the normal conditions,under nitrogen-limitation the biovolume was reduced to 30%～50% of normal and microcystin production dropped to only 10%～60%.In contrast,growth and microcystin production were not inhibited by phosphorus-limitation or even phosphorus-elimination.Nitrogen-limitation also accelerated the cell division and shortened the cell cycle.Nitrogen may influence the growth and microcystin production because it is a key element for growth and microcystin production.But,when Microcystis aeruginosa was grown under phosphorus-limited conditions,phosphorus was supplied by the phosphorus granules in the cells,so no changes were observed in the growth and microcystin production.

• Key words：Microcystis aeruginosa  nitrogen-limitation  phosphorus-limitation  growth  microcystin production

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