• 关键词：好氧反硝化  紫外诱变  碳氮比

• 基金项目：国家科技支撑项目(No.2006BAJ08B09);吉林省环保局项目(No.2008-10);吉林大学985项目

• 吕聪
• 吉林大学环境与资源学院, 长春 130026

• 马玖彤
• 吉林大学化学学院, 长春 130026

• 刘懿
• 吉林大学环境与资源学院, 长春 130026

• 张凤君
• 吉林大学环境与资源学院, 长春 130026

• 摘要：采用紫外照射的方法对好氧反硝化菌株进行诱变,从中筛选出一株优势正突变菌株ZR43,并对其形态、生长条件及反硝化能力进行了研究.结果显示,相对于亲本R1,菌株ZR43对亚硝态氮的积累量大幅度降低,从75.05mg·L^-1减少到17.42mg·L^-1.TN去除率提高了18%.菌株形态为球菌.最佳生长条件:温度为30℃、pH为8、C/N为10、以丁二酸为最佳碳源.C/N对菌株ZR43的好氧反硝化能力影响很大,当C/N大于8时,总氮的去除率均达到32%以上.

• Abstract：An aerobic denitrifier was mutagenized by UV-irradiation and a high nitrogen-removal mutant was obtained,named ZR43.The morphology,optimal culture conditions and aerobic denitrification performance of strain ZR43 were investigated.The results showed that the accumulation of NO₂^--N by the strain ZR43 was significantly less than that by the original strain R1.At nitrite concentrations from 75.05 mg·L^-1 to 17.42 mg·L^-1,the nitrogen removal rate was increased by 18% over R1.The figure of the strain ZR43 was global.The optimal culture conditions for maximum denitrification were:30℃,pH8,C/N ratio = 10,and carbon source succinic acid.The aerobic denitrification performance of strain ZR43 was influenced mostly by the C/N ratio and the nitrogen removal rate was above 32% at C/N ratios above 8.

• Key words：aerobic denitrification  UV-irradiation  C/N

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