• 关键词：镉  鸡  脾淋巴细胞  钙稳态

• 基金项目：黑龙江省教育厅科研基金项目(No.10551038)

• 肖银霞
• 辽宁医学院动物医学院, 锦州 121001

• 吉增涛
• 国家农业信息化工程技术研究中心, 北京 100097

• 李金龙
• 东北农业大学动物医学院, 哈尔滨 150030

• 徐世文
• 东北农业大学动物医学院, 哈尔滨 150030

• 摘要：为了探讨钙稳态在镉对禽类免疫抑制中的作用,以终浓度为10μmol·L^-1的CdCl₂对急性分离的鸡脾淋巴细胞进行染毒,分别在培养12、24、36、48h和72h时收集细胞,采用Fura-2/AM荧光标记、半定量RT-PCR和酶试剂盒比色的方法分别研究了细胞内游离钙离子浓度、CaMmRNA的表达以及钙泵的活性.结果表明,随着镉作用时间的延长,淋巴细胞内游离钙离子浓度不断升高,CaMmRNA表达水平和钙泵活性不断下降,试验组与对照组差异显著(p<0.05或p<0.01),提示镉可使淋巴细胞内钙稳态失调而发挥免疫毒性作用.

• Abstract：In order to investigate the role of calcium homeostasis in Cd-induced immune suppression of poultry,splenic lymphocytes isolated from chicken were exposed to 10 mol·L^-1 CdCl₂ and collected after 12 h,24 h,36 h,48 h and 72 h.The [Ca²⁺]_i,CaMmRNA levels,and the activity of the calcium pump were detected by the fluorescent dye Fura-2/AM,semi-quantitative RT-PCR and chromatometry.The results showed that as cadmium exposure time increased,the [Ca²⁺]_i gradually increased,and the CaMmRNA levels and the activity of the calcium pump gradually decreased.There were significant differences(p<0.05 or p<0.01) between the treatment groups and the control group.The results indicate that cadmium immunotoxicity arises by causing an imbalance of calcium homeostasis in lymphocytes.

• Key words：cadmium  chicken  splenic lymphocyte  calcium homeostasis

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