• 关键词：BIOLOG  细菌群落  季节动态  植被效应

• 基金项目：中国科学院西部行动计划项目(No.KZCX2-XB2-08);国家重点基础研究发展计划(973)项目(No.2006CB403208)

• 何寻阳
• 1. 中国科学院亚热带农业生态研究所, 长沙 410125; 2. 中国科学院环江喀斯特农业生态试验站, 环江 547100; 3. 中国科学院研究生院, 北京 100039

• 王克林
• 中国科学院亚热带农业生态研究所, 长沙 410125

• 徐丽丽
• 1. 中国科学院亚热带农业生态研究所, 长沙 410125; 2. 中国科学院研究生院, 北京 100039

• 陈洪松
• 1. 中国科学院亚热带农业生态研究所, 长沙 410125; 2. 中国科学院环江喀斯特农业生态试验站, 环江 547100

• 张伟
• 1. 中国科学院亚热带农业生态研究所, 长沙 410125; 2. 中国科学院环江喀斯特农业生态试验站, 环江 547100

• 摘要：基于土壤细菌代谢多样性和地上植被多样性具有协同关系的假设,利用BIOLOG技术研究西南典型喀斯特地区植被演替的不同阶段(草丛(T)、灌丛(S)、次生林(SF)和原生林(PF))土壤中细菌代谢多样性的分异特征.结果表明,植物群落和土壤细菌代谢Shannon's多样性指数分别遵循如下变化趋势:SF>PF>S>T,SF/PF>S>T.双因素方差分析结果显示,植被和植被与季节的交互效应对土壤细菌代谢Shannon's多样性有显著性影响(分别为p<0.001,p<0.05),季节变化没有显著影响(p>0.05);但同一植被下,独立样本t检验表明,细菌功能多样性仅在PF土壤中有显著的季节变化(p<0.01),而在其余土壤中季节变化不显著(p>0.05).多元方差和主成分分析表明,4种植物群落的土壤细菌利用碳源的模式具有显著差异(p<0.001);土壤细菌群落利用碳源的模式聚为3类:T、S(夏季)、SF(PF)和S(冬季).因此,植被的严重退化会降低土壤的微生物代谢多样性,而适度的人为干扰森林群落不会导致土壤细菌代谢功能的降低;灌丛土壤环境是植被演替过程中的过渡阶段,其孕育的土壤细菌具有森林阶段土壤细菌的部分特征.

• Abstract：On the basis of the hypothesis that a positive correlation exists between plant diversity and soil microbial metabolic diversity, the metabolic diversity characteristics of a soil microbial community were compared using BIOLOG_Eco microplates in different successional communities (tussock (T), shrub(S), secondary forest(SF) and primary forest(PF)) from Huanjiang County, southwest China. The values of Shannon′s diversity in the plant community were in the order: SF>PF>S>T, and soil metabolic function from highest to lowest followed the order SF/PF>S>T. Two-way ANOVA showed that both vegetation (p<0.001) and the interactions between vegetation and season (p<0.05) had significant effects on Shannon′s diversity of soil microbial metabolic diversity, but the seasonal effect itself was insignificant (p>0.05). However, for the same vegetation type, independent t-test showed that seasonal changes of microbial metabolic diversities in PFsoils were significant (p<0.01), but other types of community showed unchanged patterns. The results of MANOVA and PCAindicated that there were highly significant differences (p<0.001) in community-level physiological profiling patterns (CLPPs) among the four soil microbial communities, and CLPPs were clustered in three parts: T, S (June) and SF/PF/S (Dec). Therefore, the serious degradation of vegetation would decrease the soil microbial metabolic diversity, but the diversity of natural forest would not be affected by modest anthropogenic disturbance. The Ssoil environment, with partly similar bacterial metabolic characteristics in forest soils (SF and PF), might be a transitional stage during the process of vegetation succession.

• Key words：BIOLOG  microbial community  seasonal variation  vegetation effects

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