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环境毒理

董光辉,郑丽,金一和,逯晓波,刘洁,何钦成.全氟辛烷磺酸(PFOS)经口染毒对C57BL/6小鼠脾脏T细胞亚群CD4+/CD8+的影响[J].环境科学学报,2009,29(1):181-185

The effect of oral exposure to Perfluorooctane Sulfonate(PFOS) on spleen T cell subsets CD4+/CD8+ of C57BL/6 mice

摘要：采用每天一次经口灌胃染毒的方法,探讨了全氟辛烷磺酸(Perfluorooctanesulfonate,PFOS)经口重复剂量染毒对C57BL/6小鼠脾脏T细胞亚群CD4+和CD8+的影响.选择雄性C57BL/6小鼠24只,实验组PFOS染毒剂量分别为5、10、20mg·kg^-1(bw).每天一次经口灌胃染毒12d后,制备脾脏T淋巴细胞悬液,经特异性荧光标记的CD3(FITC)、CD4(PE)、CD8(PERCP)单克隆抗体作用后,采用FACS流式细胞仪检测T淋巴细胞及其亚群百分数.结果表明,10mg·kg^-1(bw)和20mg·kg^-1(bw)PFOS染毒组小鼠体重呈明显的下降趋势;20mg·kg^-1(bw)PFOS染毒组小鼠胸腺和脾脏指数显著低于对照组(p<0.05),而各剂量组小鼠肝脏指数均显著高于对照组(p<0.05).PFOS染毒20mg·kg^-1(bw)实验组CD3+CD4+与CD3+CD8+T细胞的绝对计数和百分比均显著低于对照组(p<0.01).研究结果显示,PFOS暴露可降低小鼠免疫器官中CD4+、CD8+T细胞的含量,表明PFOS可抑制小鼠的细胞免疫水平.

Abstract：The objective of this experiment was to assess the effects of Perfluorooctane Sulfonate(PFOS) on spleen Tcell subsets through detecting the average positive rate and the numbers of splenic subpopulations of CD4+ and CD8+ Tcells.Twenty-four adult C57BL/6 male mice were divided into four groups.Animals were given0,5,10 and 20 mg·kg^-1 PFOSby oral gavage every day for 12 days,and then single-cell suspensions of splenocytes were prepared.After labeling with the Fluorescein Isothiocyanate(FITC) conjugated rat-anti-mouse CD3(monoclonal),phycoerythrin(PE) conjugated rat anti-mouse CD4(monoclonal) and peridinin chlorophyl protein(Percp) conjugated rat anti-mouse CD8(monoclonal),the phenotypes of splenocyte populations were analyzed by Flow Cytometry(FCM).The results showed that in the 12-d study,body weight of mice exposed to 10 mg·kg^-1 and 20 mg·kg^-1 of PFOSshowed significant deterioration from their own pre-exposed baseline.Spleen and thymus mass were significantly decreased compared to the control following exposure to 20 mg·kg^-1 of PFOS.Furthermore,liver mass was increased in each exposure group.Flow cytometric analysis showed that the average positive rate and the numbers of splenic subpopulations of CD4+ and CD8+ cells decreased significantly in the 20 mg·kg^-1 PFOSgroup in comparison with normal C57BL/6 mice.This study indicates that PFOSmay decrease the average positive rate of CD4+ and CD8+T-cell in spleen organs,thus suppressing immune function.