• 关键词：生物表面活性剂  分离鉴定  发酵工艺优化  生物修复

• 基金项目：国家自然科学基金面上项目(No.30870081,20677027)

• 曹娟
• 南京农业大学生命科学学院, 农业部农业环境微生物工程重点开放实验室, 南京 210095

• 刘怡辰
• 南京农业大学生命科学学院, 农业部农业环境微生物工程重点开放实验室, 南京 210095

• 张振华
• 南京农业大学生命科学学院, 农业部农业环境微生物工程重点开放实验室, 南京 210095

• 冉炜
• 南京农业大学资源和环境科学学院, 江苏省固体废弃物资源化高技术研究重点实验室, 南京 210095

• 沈标
• 南京农业大学资源和环境科学学院, 江苏省固体废弃物资源化高技术研究重点实验室, 南京 210095

• 摘要：从石油污染土壤中分离筛选获得一株产生生物表面活性剂菌株Y8A,经生理生化实验、16S rDNA序列分析等将其鉴定为芽孢杆菌属(Bacillus sp.).Y8A能在22h内将发酵液的表面张力从68.3mN·m^-1降到23.5mN·m^-1.经TLC和傅立叶红外光谱分析,菌株Y8A产生的生物表面活性剂为脂肽类.20mg·L^-1Ca²⁺和Fe₂₊能显著促进其生长和表面活性剂的产生;菌株Y8A在20～30℃,pH5～12范围内产生表面活性剂的能力较强;LB培养基中添加1%乳糖对生长的影响不大,但能够明显促进Y8A产生生物表面活性剂,而葡萄糖、蔗糖抑制表面活性剂的产生.Y8A能够促进石油降解菌Y1D和F1-1对石油的降解和功夫菊酯降解菌ZZH对功夫菊酯的生物降解.

• Abstract：A biosurfactant-producing bacterium Y8A was isolated from the petroleum-polluted soil. Strain Y8A was identified as Bacillus sp. according to its morphological features,physiological and biochemical characteristics and 16S rDNA sequences. Strain Y8A could decrease the surface tension of the medium from 68.3 mN·m^-1 to 23.5 mN·m^-1 after 22h incubation. The biosurfactant produced by strains Y8A was identified as lipopeptide by TLC and Fourier transform infrared spectroscopy (FTIR). 20mg·L^-1 Fe₂₊ and Ca²⁺ could promote the growth of Y8A and production of biosurfactant. Addition of 1% lactose in LB medium was helpful to the production of biosurfactant by strain Y8A although no obvious promotion to the growth was observed. Strain Y8A grew better and produced more biosurfactant at 20～30℃,pH 5～12.Y8A could enhance the biodegradation of petroleum-oil by Y1D and F1-1 and the biodegradation of insecticide by ZZH.

• Key words：biosurfactant  isolation and identification  fermentation condition optimizing  bioremediation

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