污染控制技术及原理
王蕾,聂麦茜,王志盈,惠艳,玉亚,徐会宁.一株芽胞杆菌和一株黄杆菌代谢芘的摄取方式解析[J].环境科学学报,2009,29(5):924-929
一株芽胞杆菌和一株黄杆菌代谢芘的摄取方式解析
- Mode of metabolic pyrene uptake by a Bacillus sp. and a Flavobacterium sp.
- 基金项目：陕西省教育厅专项科研计划项目(No.07JK290);陕西省自然科学基金项目(No.2006Z04)
- 作者
- 单位
- 王蕾
- 西安建筑科技大学环境与市政工程学院, 西安 710055
- 聂麦茜
- 西安建筑科技大学环境与市政工程学院, 西安 710055
- 王志盈
- 西安建筑科技大学环境与市政工程学院, 西安 710055
- 惠艳
- 西安建筑科技大学环境与市政工程学院, 西安 710055
- 玉亚
- 西安建筑科技大学环境与市政工程学院, 西安 710055
- 徐会宁
- 西安建筑科技大学环境与市政工程学院, 西安 710055
- 摘要：通过溶剂萃取、薄层色谱和紫外分光光度法研究了一株芽胞杆菌CN2和一株黄杆菌CN4在初始pH=6.00的条件下对芘的降解过程.研究表明:芘降解液的pH值随降解时间向中性波动变化;用牛肉膏蛋白胨培养出的CN2菌体表面具有亲水性,CN4菌体表面具有疏水性,CN2表面疏水性在芘的降解过程中随时间有变大的趋势,CN4表面疏水性在芘的降解过程中随时间有变小的趋势;从芘降解的整个过程看,大部分时间CN2的降解比CN4的快,CN4菌体捕获的芘量比CN2要大,尤其在接种刚开始的一段时期较为明显;细胞表面、细胞壁中、细胞内部均有芘富集迹象,其中细胞表面富集的芘最多.根据试验结果推测,在菌株对芘的降解过程中吸附和降解同时发生;菌体对芘首先通过表面吸附或其它方式结合,随后溶入细胞壁中,跨过细胞膜进入细胞内被降解;这个传质过程很可能不是连续的,而是由细菌控制的主动的间歇的动态过程.
- Abstract：The pyrene biodegradation by Bacillus sp.CN2 and Flavobacterium sp.CN4 was investigated by carefully designed solvent extraction experiments,thin layer chromatography(TLC)and UV-spectrophotometry The pHvalue of the culture media increased from 6.00 to neutral with continuous fluctuations.Cultured in peptone beef medium,the cell surface of CN2 was with strong hydrophilic and that of CN4 was with strong hydrophobic.The cell surface hydrophobicity(CSH)of CN2 increased while the CSHof CN4 decreased during the pyrene biodegradation process.Pyrene biodegradation by CN2 was faster than that by CN4.The total amount of pyrene captured by CN2 was less than that by CN4,especially in the initial inoculation period.It was also shown that pyrene was present on the cell surface,within the cell wall and inside the cells,though the largest amount of pyrene was on the surface of the cells.The pyrene captured by the cells resisted vigorous shaking during water rinsing and withstood cyclohexane extraction from aqueous solution.It is possible that pyrene sorption and biodegradation are concurrent and the adsorbed pyrene on the cell surfaces can ultimately be transferred into cells and decomposed and oxidized there.The mass transfer of pyrene from culture fluids onto the cell surfaces,then through cell walls and membranes into cells may not be continuous,but is possibly controlled by the microbe itself.
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