• 关键词：苯酚  复合菌群  生物降解  动力学

• 基金项目：湘潭市科技计划重点项目(No.ZD20081027)

• 姜和
• 湘潭大学化工学院, 生物技术研究所, 湘潭 411105

• 曾虹燕
• 湘潭大学化工学院, 生物技术研究所, 湘潭 411105

• 夏葵
• 湘潭大学化工学院, 生物技术研究所, 湘潭 411105

• 黄炎
• 湘潭大学化工学院, 生物技术研究所, 湘潭 411105

• 廖凯波
• 湘潭大学化工学院, 生物技术研究所, 湘潭 411105

• 陈良
• 湘潭大学化工学院, 生物技术研究所, 湘潭 411105

• 摘要：为了获得能降解苯酚的高效微生物菌群,研究了不同条件(温度、pH、接种量、振荡速率及初始苯酚浓度等)对复合菌群JHD降酚性能的影响.结果表明,32℃、pH=7.0、接种量为10%和振荡速率为150r·min^-1,初始苯酚浓度为1000mg·L^-1时降解苯酚16h,降酚率高达99.97%,残余苯酚浓度低于0.28mg·L^-1,远低于国家一级排放标准.采用Andrews方程对复合菌群JHD降解苯酚的过程进行拟合,其动力学参数为q_max=0.41h^-1,K_s=55.44mg·L^-1,K_i=970.06mg·L^-1,复合菌群JHD降解苯酚的最佳初始苯酚浓度为231.90mg·L^-1,实验数据与该动力学方程拟合较好.

• Abstract：In order to obtain a mixed culture with high efficiency in phenol-degradation,the phenol-degradation conditions of mixed culture JHD,including temperature,pH,inoculum size,agitation rate and initial phenol concentration,were systematically studied.The results showed that under the conditions of pH= 7,32℃,inoculum size 10% and agitation rate 150 r·min^-1 within 16 h,the phenol concentration was rapidly reduced from 1000 to below 0.28 mg·L^-1 with the phenol-degradable rate higher than 99.97%.The residual phenol concentration was well below the requirement of first grade discharge standard in China.The degradation kinetic data fitted the Andrews model well.The calculated parameters of q_max,K_s and K_i for the model were 0.41 h^-1,55.44 mg·L^-1 and 970.06 mg·L^-1,respectively,and the optimal concentration of initial phenol was 231.90 mg·L^-1.

• Key words：phenol  mixed culture  biodegradation  kinetics

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