污染控制技术及原理
张隽,黄星,邱吉国,李顺鹏,何健.氟铃脲降解菌FLN-1的分离鉴定及降解特性[J].环境科学学报,2009,29(6):1178-1183
氟铃脲降解菌FLN-1的分离鉴定及降解特性
- Isolation and identification of hexaflumuron-degrading bacterium FLN-1 and its degradation characteristics
- 基金项目:国家高技术研究发展计划(863)项目(No.2006AA10Z402);国家自然科技平台项目(No.2005DKA21201-2)
- 张隽
- 南京农业大学生命科学学院, 农业部农业环境微生物工程重点开放实验室, 南京 210095
- 黄星
- 南京农业大学生命科学学院, 农业部农业环境微生物工程重点开放实验室, 南京 210095
- 邱吉国
- 南京农业大学生命科学学院, 农业部农业环境微生物工程重点开放实验室, 南京 210095
- 李顺鹏
- 南京农业大学生命科学学院, 农业部农业环境微生物工程重点开放实验室, 南京 210095
- 何健
- 南京农业大学生命科学学院, 农业部农业环境微生物工程重点开放实验室, 南京 210095
- 摘要:从农药厂废水处理池的活性污泥中分离到1株能降解氟铃脲的菌株,命名为FLN-1.根据表型特征、生理生化特性及16S rDNA序列同源性分析,将FLN-1初步归类为红球菌属(Rhodococcus sp.).研究结果表明,该菌能在含氟铃脲(50mg·L-1)的基础盐液体培养基中降解氟铃脲,5d降解率达85%,降解最适pH为6.0~9.0,最适温度为25~40℃,降解速率随初始接种量的增加而增大;100mg·L-1的葡萄糖、酵母膏和蛋白胨对菌株降解氟铃脲具有促进作用.酶的定域试验表明,降解氟铃脲的酶为胞内酶.
- Abstract:A hexaflumuron-degrading bacterium,FLN-1,was isolated from activated sludge collected from the wastewater-treatment system of a pesticide factory.Based on the analysis of its 16S rDNA,morphology and physiological characteristics,strain FLN-1 was identified as Rhodococcus sp.This strain could degrade 85% of 50 mg·L-1 hexaflumuron within 5 days.The optimum pH and temperature ranges for the degradation were 6.0~9.0 and 25~40℃,respectively.The degradation efficiency was positively correlated to initial inoculum size.Addition of 100 mg·L-1 yeast extract,glucose or peptone could enhance the hexaflumuron degradation rate.Enzyme distribution experiment results indicated that the hexaflumuron degrading enzyme of strain FLN-1 was an endoenzyme.
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