污染控制技术及原理
李春艳,李艳春,成小松,肖晶,熊明华,潘俊波.一株丁草胺降解菌的分离鉴定及培养条件优化[J].环境科学学报,2010,30(2):347-353
一株丁草胺降解菌的分离鉴定及培养条件优化
- Isolation and identification of a butachlor degrading bacterium and optimization of its growth conditions
- 基金项目：东北农业大学创新团队基金(No.CXT003-1-3);黑龙江省教育厅科学技术研究项目(No.11541015)
- 作者
- 单位
- 李春艳
- 东北农业大学资源与环境学院, 哈尔滨 150030
- 李艳春
- 东北农业大学资源与环境学院, 哈尔滨 150030
- 成小松
- 哈尔滨医科大学第一临床医学院, 哈尔滨 150001
- 肖晶
- 东北农业大学资源与环境学院, 哈尔滨 150030
- 熊明华
- 东北农业大学资源与环境学院, 哈尔滨 150030
- 潘俊波
- 东北农业大学资源与环境学院, 哈尔滨 150030
- 摘要：利用富集培养技术从长期施用丁草胺的稻田土壤中分离得到1株能够降解丁草胺的细菌,标记为LYC-2.经形态特征、生理生化特征和16SrDNA序列分析,将该菌株鉴定为钩杆菌属(Ancylobactersp.).同时,为探索菌株LYC-2的生长特性和最佳培养条件,利用正交试验设计法考察了接种量、温度、pH值3个因素对菌株LYC-2生长的影响.结果表明,菌株LYC-2的最适生长温度为35℃,最适pH值为7.5.当接种量为6%(体积分数,下同)时,该菌株在含100mg·L-1的丁草胺无机盐基础培养液中培养5d后,可使丁草胺降解率达90.85%以上.
- Abstract：Abutachlor-degrading strain LYC-2 was isolated from a paddy soil sample by enrichment culture.According to morphologic and physiobiochemical characteristics as well as 16SrDNAsequence analysis,strain LYC-2 was identified as an Ancylobacter sp.Effects of inoculation rate (V/V),temperature and pHon the growth of the butachlor-degrading strain were examined using an orthogonal experiment.The results showed that the optimal inoculation rate was 6% (V/V) in mineral medium containing 100mg·L-1 butachlor,at 35℃ and pH7.5 Under these conditions,more than 90.85% butachlor was degraded by LYC-2 in 5 days.
- Key words：butachlor Ancylobacter sp. isolation and identification growth characteristics cultural conditions orthogonal experiment
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