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环境毒理

Oxidative DNA damage in rat liver cells induced by formaldehyde at different concentrations

摘要：为了探讨外源性化合物暴露致内脏细胞DNA氧化损伤程度的定量检测方法,尝试以8-羟基脱氧鸟苷(8-hydroxy-2'-deoxyguanosine,8-OHdG)为分子生物标志物,以甲醛为模式外源性化合物,以丙二醛(malondialdehyde,MDA)为参考指标,应用大鼠肝细胞悬液进行体外染毒实验研究.同时,实验设置甲醛染毒的终浓度分别为0、5、15、45μmol·L^-1,在大鼠肝细胞悬液染毒1h后,分别测量了肝细胞悬液中的8-羟基脱氧鸟苷(8-OHdG)和丙二醛(MDA)含量.研究结果显示,随着甲醛浓度升高,大鼠肝细胞中8-OHdG和MDA含量均呈升高趋势(F=59.55,p<0.01;F=75.82,p<0.01),高浓度组(45μmol·L^-1)8-OHdG和MDA含量与对照组的差异均具有显著性(p<0.01),中浓度组(15μmol·L^-1)的这种差别也具有显著性(p<0.05,p<0.01),低浓度组(5μmol·L^-1)的这种差异没有显著性(p>0.05).因此,8-OHdG不但可以用于血液和尿液样品的检测,而且采用本项研究摸索出的前处理方法可以很好地定量测定内脏细胞DNA氧化损伤的程度.

Abstract：We developed a quantitative detection method that can reflect the degree of visceral cell oxidative DNA damage induced by exogenous compound exposures using 8-OHdG as a molecular biomarker in vitro.In this study formaldehyde was used as a model toxic compound and malondialdehyde(MDA)was used as a reference indicator.Rat liver cells were exposed to different concentrations of formaldehyde(0,5,15,45μmol·L^-1)for 1 h,and then the contents of 8-OHdG and MDA were measured.Both 8-OHdG and MDA increased gradually with the increase of formaldehyde concentration(F=59.55,p<0.01;F=75.82,p<0.01).At high formaldehyde levels(45μmol·L^-1)the 8-OHdG and MDA contents were extremely significantly different when compared with the control group(p<0.01),at intermediate formaldehyde levels(15μmol·L^-1)their differences were significant(p<0.01,p<0.05),and at the low level(5μmol·L^-1)there was no significant difference(p>0.05).We conclude that 8-OHdG can be used not only for blood and urine sample testing,but also as a good biomarker oxidative DNA damage,in visceral cells.