污染控制技术及原理
顾平,张倩茹,周启星,张颖,杨肖娥.一株苯并[a]芘高效降解真菌的筛选与降解特性[J].环境科学学报,2010,30(2):354-360
一株苯并[a]芘高效降解真菌的筛选与降解特性
- Isolation of a fungal strain capable of degrading benzo [a] pyrene and its degradation characteristics
- 关键词：污染土壤 苯并[a]芘(B[a]P) 绿色木霉 生物修复 降解作用
- 基金项目：国家高技术研究发展计划(863)项目(No.2006AA06Z386);污染环境修复与生态健康教育部重点实验室开放基金(No.EREH0710);教育部科技创新工程重大项目培育资金项目(No.707011)
- 作者
- 单位
- 顾平
- 1. 东北农业大学资源与环境学院, 哈尔滨 150030; 2. 中国科学院沈阳应用生态研究所, 中国科学院陆地生态过程重点实验室, 沈阳 110016
- 张倩茹
- 中国科学院沈阳应用生态研究所, 中国科学院陆地生态过程重点实验室, 沈阳 110016
- 周启星
- 中国科学院沈阳应用生态研究所, 中国科学院陆地生态过程重点实验室, 沈阳 110016
- 张颖
- 东北农业大学资源与环境学院, 哈尔滨 150030
- 杨肖娥
- 浙江大学环境与资源学院, 污染环境修复与生态健康教育部重点实验室, 杭州 310029
- 摘要：从长期受多环芳烃(PAHs)污染的土壤中分离出一株能够降解苯并[a]芘(B[a]P)的真菌,经鉴定为绿色木霉(Trichoderma viride)(命名为BF-1),并对其以B[a]P为唯一碳源进行反复驯化,考察了B[a]P浓度、不同重金属和培养基对其降解能力的影响.结果表明,菌株BF-1在B[a]P浓度为5mg·L-1,32℃振荡培养约6d的条件下,降解速度最快,B[a]P的降解率达68.28%.BF-1在B[a]P浓度分别为10与25mg·L-1,32℃振荡培养6d的条件下,B[a]P的降解率分别为73.29%与87.36%.Cu2+(50mg·L-1)基本不影响BF-1对B[a]P的降解率;Cd2+(100mg·L-1)、Pb2+(300mg·L-1)对BF-1降解B[a]P有一定影响,但仍表现出较高的耐受能力;而Zn2+(200mg·L-1)对BF-1有明显的抑制作用.选用含5mg·L-1B[a]P的土豆葡萄糖液体培养基,6d后B[a]P的降解率为71.31%.对比前述实验结果表明,培养基对B[a]P降解率的影响并不明显.因此,BF-1的应用价值较高.
- Abstract：Afungal strain,BF-1,with optimal degradation ability for the polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (B[a]P) was isolated from long-term PAH-contaminated soil.The strain was identified as Trichoderma viride.The effects of B[a]Pconcentration,various heavy metals and culture media on the degradation ability of the fungal strain were further studied using repeated subcultures in liquid medium with B[a]Pas the only carbon source.The highest degradation rate of 5 mg·L-1 B[a]Pby BF-1 was 68.28% after a 6-day culture at 32 ℃.After a 6-day oscillating culture at 32 ℃,the degradation rates of 10 and 25 mg·L-1 B[a]Pin the culture medium were 73.29% and 87.36%,respectively.Addition of Cu2+(50 mg·L-1) could appreciably inhibit the biodegradation of B[a]Pby BF-1,and Pb2+ (300 mg·L-1) and Cd2+ (100 mg·L-1) also affected the rate of degradation,but the strain had strong tolerance to Pb2+ (300 mg·L-1) or Cd2+ (100 mg·L-1 ).In particular,there was an obvious inhibitory effect of Zn2+ (200 mg·L-1) on the rate of degradation.After inoculation in potato dextrose broth culture media,BF-1 could degrade 5 mg·L-1 B[a]Pat a rate of 71.31% in6 days,and compared with the results above,it was concluded that the culture media had minimal effect on the degradation of B[a]P.Therefore,fungal strain BF-1 should be valuable in degrading B[a]Pbecause it can tolerate a wide range of culture conditions.
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