污染控制技术及原理
张楠,刘东阳,杨兴明,徐阳春,沈其荣,黄启为.分解纤维素的高温真菌筛选及其对烟杆的降解效果[J].环境科学学报,2010,30(3):549-555
分解纤维素的高温真菌筛选及其对烟杆的降解效果
- Screening of thermophilic cellulose-decomposing fungi and their efficiency in decomposing tobacco stems
- 基金项目：农业部农业公益性行业科研专项(No200803031);农业部948项目(No2006-G62)
- 作者
- 单位
- 张楠
- 南京农业大学资源与环境科学学院, 南京 210095
- 刘东阳
- 南京农业大学资源与环境科学学院, 南京 210095
- 杨兴明
- 南京农业大学资源与环境科学学院, 南京 210095
- 徐阳春
- 南京农业大学资源与环境科学学院, 南京 210095
- 沈其荣
- 南京农业大学资源与环境科学学院, 南京 210095
- 黄启为
- 南京农业大学资源与环境科学学院, 南京 210095
- 摘要：为研制促进烟杆堆肥的微生物菌剂,进行了分解纤维素的高温真菌筛选.结果表明,从不同原料腐熟堆肥中筛选到4株降解纤维素的高温真菌,它们均能在以羧甲基纤维素钠(CMC-Na)或烟杆粉末为唯一碳源的培养基上生长.菌株在2～5d内可长满CMC-Na-刚果红平板和烟杆粉末培养基.菌株摇瓶培养时2d可达到产酶高峰,酶活(CMCase)超过10U.菌株对烟杆的降解效果较好,7d内的降解率最高可达42.2%,对烟杆中的纤维素、半纤维素和木质素的分解率分别可达52.7%、47.9%和37.6%.
- Abstract：Thermophilic cellulose-decomposing fungi were screened to develop microbial inoculums which could promote the composting of tobacco stems.Four strains of thermophilic cellulose-decomposing fungi were isolated from composts of different substrates,and they could use carboxymethyl cellulose Na(CMC-Na) or tobacco stem powder as sole carbon source.The strains could overgrow in CMC Na-Congo red medium and tobacco stem powder medium within 2～5 days.In liquid medium,the strains produced the highest CMCase activity after 2 days inoculation,which reached 10U.They could decompose tobacco stems,cellulose,hemicellulose and lignin in the tobacco stems by 42.2%,52.7%,47.9% and 37.6%,respectively,within 7 days.
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