• 关键词：亚硝化/电化学反硝化  细菌  多样性  16S rDNA  Proteobacteria类群

• 基金项目：国家自然科学基金项目(No.50708120);国家高技术研究发展计划(863)项目(No.2004AA649320)

• 王海燕
• 中国环境科学研究院水污染控制技术研究中心, 北京 100012

• 周岳溪
• 中国环境科学研究院水污染控制技术研究中心, 北京 100012

• 刘海涛
• 1. 中国环境科学研究院水污染控制技术研究中心, 北京 100012; 2. 北京华利嘉环境工程技术有限公司, 北京 100022

• 戴欣
• 中国科学院微生物研究所, 北京 100080

• 张伟华
• 1. 中国环境科学研究院水污染控制技术研究中心, 北京 100012; 2. 湖南农业大学资源环境学院, 长沙 410128

• 曾清如
• 湖南农业大学资源环境学院, 长沙 410128

• 摘要：采用电镜观察和分子生物学手段16SrDNA克隆文库方法对亚硝化/电化学生物反硝化全自养脱氮工艺中的细菌进行了形态和多样性研究,从16SrDNA克隆文库中随机挑选60(61)个克隆子进行序列测定(约500bp),对测序结果进行BLAST比对和系统发育分析.结果表明,亚硝化段内的细菌主要为球状和椭球状的氨氧化细菌,以亚硝酸氮作为进水基质时,电化学反硝化生物段内细菌主要为短杆状和椭球状的脱氮菌.亚硝化/电化学生物反硝化脱氮系统中蕴藏着特有的微生物新资源.亚硝化段细菌类群的优势顺序为β-Proteobacteria类群(60.00%)、Bacteroidetes类群(28.33%)和Chloroflexi类群(11.67%).当电化学生物反硝化段进水氮基质为亚硝氮(429～543mg·L^-1)和氨氮(412～525mg·L^-1)时,细菌优势类群顺序为β-Proteobacteria(78.33%)类群和ε-Proteobacteria类群(21.67%);当电化学生物反硝化段进水氮基质为亚硝氮(519～578mg·L^-1)时,细菌优势类群顺序为β-Proteobacteria类群(81.97%)、ε-Proteobacteria(16.39%)类群和γ-Proteobacteria类群(1.64%);优势类群变化不大,但每种类群中细菌的种类和数量变化较大,这主要是由进水基质变化导致.

• Abstract：The morphology and diversity of the bacteria in the complete autotrophic nitrite-nitrification and electrochemical bio-denitrification combined process were studied by scanning electron microscopy (SEM) and molecular biological methods,i.e.partial 16SrDNAcloning and sequencing.Sixty (61) clones were randomly selected,and their partial 16SrDNAgene (ca.500 bp) was sequenced and searched in the BLASTdatabase.Arepresentative clone of each phylotype was also phylogenetically analyzed.The dominant bacteria in the nitrite-nitrification phase were ammonium oxidizing bacteria in spherical and elliptical form,while the short-bacilliform and elliptical nitrogen removing bacteria were the dominant bacteria in the electrochemical denitrification phase when nitrite-nitrogen was the influent nitrogen substrate.Some unknown bacteria with new characteristics were found in the combined process.The dominant bacterial communities in the nitrite-nitrification phase were β-Proteobacteria (60.00%),Bacteroidetes (28.33%) and Chloroflexi (11.67%).The dominant communities in the electrochemical bio-denitrification phase were β-Proteobacteria (78.33%) and ε-Proteobacteria (21.67%) when the influent ammonia and nitrite-nitrogen concentrations were 429～543 mg·L^-1 and 412～525 mg·L^-1.When₅₁₉～578 mg·L^-1 nitrite was the only nitrogen substrate in the influent,the dominant communities of the electrochemical bio-denitrification phase,which changed slightly,were β-Proteobacteria (81.97%),ε-Proteobacteria (16.39%) andγ-Proteobacteria(1.64%).However,the species and amount of the bacteria in each community changed relatively greatly as a result of the change of influent substrate.

• Key words：nitrite-nitrification and electrochemical bio-denitrification  bacterial diversity  16S rDNA  Proteobacteria

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