欢迎访问《环境科学学报》编辑部网站！

快速检索			高级检索

污染控制技术及原理

摘要：链霉菌(Streptomyces sp.)HJ02能以吲哚为唯一碳源生长,考察了初始吲哚质量浓度、外加碳源、温度、pH等对其降解效果的影响,探讨了该菌降解吲哚的动力学与机理.结果表明,链霉菌对吲哚有很强的耐受力,在pH=7、温度为30℃的条件下培养6d,初始浓度为300mg·L^-1吲哚的降解率达到97.4%.菌株对吲哚的降解过程动力学符合Andrews方程.外加葡萄糖会抑制链霉菌降解吲哚的速率,葡萄糖与吲哚之间存在底物竞争抑制.紫外光谱与GC分析表明,吲哚的特征杂环被链霉菌破坏.

Abstract：Streptomyces sp. HJ02 can use indole as the sole carbon source for growth. The effects of initial concentration of indole,added glucose,pHand temperature on the biodegradation of indole by Streptomyces sp. HJ02 were examined. The main metabolic pathways and mechanism of indole degradation by HJ02 strain are discussed. The results showed that Streptomyces sp. HJ02 has a rather strong capacity to biodegrade indole. The Streptomyces sp. HJ02 degraded nearly 300 mg·L^-1 of indole within 6 days under the optimum conditions of pH=7,and 30℃. The degradation kinetics of indole by Streptomyces sp. HJ02 could be fitted adequately to an Andrews equation. Adding glucose decreased the degradation rate of indole by Streptomyces sp. HJ02,showing competitive inhibition between glucose and the indole substrate. The ultraviolet-visible spectra and GCchromatogram showed that the characteristic ring of indole was broken down by Streptomyces sp.