• 关键词：聚乙烯醇降解酶  Plackett-Burman design  响应面  优化

• 基金项目：湖南省教育厅资助科研项目(No.09C380)

• 王能强
• 湖南科技大学生命科学学院, 湘潭 411201

• 赵维俊
• 湖南科技大学生命科学学院, 湘潭 411201

• 梁芳
• 湖南科技大学生命科学学院, 湘潭 411201

• 李会东
• 湖南科技大学生命科学学院, 湘潭 411201

• 王栋
• 湖南科技大学生命科学学院, 湘潭 411201

• 摘要：为了提高假单胞菌Pseudomonas.spXT11-Z90S产聚乙烯醇降解酶的能力,用Plackett-Buman实验和响应面分析实验对影响产聚乙烯醇降解酶的9个因素,即发酵培养基的9种组成成分进行了研究,并探讨了影响产酶的主要因素和培养基的最佳组成.结果表明,影响产聚乙烯醇降解酶的3个重要组成为葡萄糖、酵母膏和硫酸铵,其最适浓度分别为0.64、0.22和1.45g·L^-1.在最佳浓度和组成条件下,聚乙烯醇降解酶最高酶活为13.5U·mL^-1,与优化前的12.1U·mL^-1相比,提高了11.6%,且其降解率6h时可达到35.5%.

• Abstract：Two level factorial designs of the Plackett-Burman type were constructed to optimize the fermentation medium components of a poly(vinyl alcohol)-degrading enzyme produced by strain Pseudomonas sp.XT11-Z90S.The results showed that the concentrations of glucose,yeast extract and(NH₄)₂SO₄ greatly influence the activity of poly(vinyl alcohol)-degrading enzyme.The optimum concentrations of glucose,yeast extract and(NH₄)₂SO₄,determined by response surface methodology,were 0.64,0.22 and 1.45 g·L^-1,respectively.Under the optimal conditions mentioned above,the poly(vinyl alcohol)-degrading enzyme activity is 13.5U·mL^-1,which was increased by 11.6% compared with the value before optimization.

• Key words：poly(vinyl alcohol)-degrading enzyme  Plackett-Burman design  response surface methodology  optimization

• 
  摘要点击次数： 3053 全文下载次数： 3037