• 关键词：焦亚硫酸钠  二氧化硫  大鼠背根节神经元  全细胞膜片钳技术  L-型钙电流

• 基金项目：国家自然科学基金资助项目(No.20677035,20477023);山西省自然科学基金(No.20031092)

• 魏彩玲
• 1. 长治医学院预防医学教研室, 长治 046000; 2. 山西大学环境医学与毒理学研究所, 太原 030006

• 孟紫强
• 山西大学环境医学与毒理学研究所, 太原 030006

• 摘要：为了探讨二氧化硫(SO₂)及其衍生物对背根节神经元的生理调节作用,应用全细胞膜片钳技术,研究了SO₂衍生物焦亚硫酸钠(sodiummetabisulfite,SMB)对大鼠背根节神经元L-型钙电流(I_Ca,L)的影响.结果表明,SMB可增大ICa,L,且具有剂量依赖性和电压依赖性的特征.SMB可使I_Ca,L的激活和失活过程均向去极化方向移动,但对失活的影响要大于对激活的影响,从而可导致背根节神经元钙离子内流增大,引起细胞内钙离子浓度增加.这说明SO₂及其衍生物对背根节神经元的生理功能有调节作用,当其浓度过高时也可能引起该神经元的病理生理改变.

• Abstract：To explore the regulating role of SO₂ and its derivatives on the physiological function of dorsal root ganglion(DRG)neurons,the effect of sodium metabisulfite(SMB),a common sulfur dioxide(SO₂)derivative and food preservative,on the L-type calcium current(I_Ca,L)of rat DRGneurons was studied using the whole cell configuration by patch-clamp technique.The present results show that SMBincreased the I_Ca,L in a concentration-and voltage-dependent manner.SMB shifted both the steady-state activation and the inactivation curves of I_Ca,Lto more positive potentials,and the shift of the inactivation curves was more pronounced than that of the activation curves.The increase of I_Ca,Lby SMB might cause an increase of intracellular calcium concentration via voltage-gated L-type calcium channels.These results lead to the conclusion that SO₂ and its derivatives could regulate the physiological function of DRGneurons,and these chemicals at high concentrations could also cause pathophysiologic changes of the neurons.

• Key words：sodium metabisulfite  sulfur dioxide  rat dorsal root ganglion neurons  whole-cell patch-clamp technique  L-type calcium current

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