污染控制技术及原理
皇甫鑫,廖翀,杨坪,钱蜀,张丹,方维凯,但德忠.金属化合物对发光菌的毒性效应及不同发光菌的敏感度差异研究[J].环境科学学报,2010,30(9):1787-1792
金属化合物对发光菌的毒性效应及不同发光菌的敏感度差异研究
- Toxicity of metal compounds to luminescent bacteria and sensitivity differences of different luminescent bacteria
- 基金项目：
- 作者
- 单位
- 皇甫鑫
- 四川大学建筑与环境学院, 成都 610065
- 廖翀
- 四川省环境监测中心站, 成都 610041
- 杨坪
- 四川省环境监测中心站, 成都 610041
- 钱蜀
- 四川省环境监测中心站, 成都 610041
- 张丹
- 四川省环境监测中心站, 成都 610041
- 方维凯
- 四川大学建筑与环境学院, 成都 610065
- 但德忠
- 四川大学建筑与环境学院, 成都 610065
- 摘要：利用发光菌毒性测试技术,研究了5种重金属化合物HgCl2、Pb(NO3)2、Cd(NO3)2、K2Cr2O7、NaAsO2对3种常见发光菌种——青海弧菌Q67、明亮发光杆菌502、费氏弧菌的毒性效应差异.同时,对发光抑制率和金属化合物浓度进行线性回归分析后计算了EC50值(半数效应浓度值),并对比了不同菌种对各金属化合物的敏感度差异及特点.结果表明,5种重金属对青海弧菌Q67的毒性大小顺序为HgCl2>Cd(NO3)2>NaAsO2>Pb(NO3)2>K2Cr2O7;对明亮发光杆菌502的毒性大小顺序为:HgCl2>NaAsO2>Pb(NO3)2>Cd(NO3)2>K2Cr2O7;对费氏弧菌的毒性大小顺序为:HgCl2>Pb(NO3)2>Cd(NO3)2>NaAsO2>K2Cr2O7.研究结果对利用发光菌毒性测试技术测定不同废水污染时菌种的选择具有指导作用,在未知污染物的情况下应选用几种菌种同时检测,这样可以保证检测结果的可靠性.
- Abstract：The inhibiting toxicity of five heavy metal compounds-HgCl2,Pb(NO3)2,Cd(NO3)2,K2 Cr2 O7 and NaAsO2-on the luminescence of three kinds of luminescent bacteria-Vibrio qinghaiensis sp.-Q67,Photobacterium phosphoreum and Vibrio fischeri-was investigated by use of the Microtox test.The EC50 s(Median Effective Concentrations) of the five metals were measured based on linear regression analysis.The results showed that the toxic effects of the heavy metal compounds on the three kinds of luminescent bacteria were different.The toxicity order among the five heavy metal compounds to Vibrio qinghaiensis sp.-Q67 was HgCl2 > Cd(NO3)2 > NaAsO2 > Pb(NO3)2 > K2 Cr2 O7.To Photobacterium phosphoreum the toxicity order was HgCl2 > NaAsO2 > Pb(NO3)2 > Cd(NO3)2 > K2 Cr2 O7,and to Vibrio fischeri the order was HgCl2 > Pb(NO3)2 > Cd(NO3)2 > NaAsO2 > K2 Cr2 O7.The research results are an important guide to determine the risk of different wastewaters.The choice of different luminescent bacteria depends on the kind of wastewater tested by the Microtox test.If unknown pollutants are present in the wastewater,several kinds of luminescent bacteria should be chosen to ensure the reliability of results by Microtox test.
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