• 关键词：丛枝菌根真菌(AMF)  镉  钙  钙离子通道阻断剂  三叶草(Trifolium repense L.)

• 基金项目：国家重点基础研究发展计划(973)前期研究专项(No.2008CB417207)

• 黄永恒
• 广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广东省微生物应用新技术公共实验室, 广州 510070

• 姚青
• 华南农业大学园艺学院, 广州 510642

• 郭俊
• 广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广东省微生物应用新技术公共实验室, 广州 510070

• 龙良鲲
• 广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广东省微生物应用新技术公共实验室, 广州 510070

• 杨瑞恒
• 广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广东省微生物应用新技术公共实验室, 广州 510070

• 朱红惠
• 广东省微生物研究所, 广东省菌种保藏与应用重点实验室, 广东省微生物应用新技术公共实验室, 广州 510070

• 摘要：本试验以三叶草(Trifoliumrepense L.)为宿主植物,Glomus intraradices为供试菌种,通过分室系统将外生菌丝与植物根系分隔开,并对菌丝室加以不同含量水平的Ca²⁺、Cd²⁺和Ca²⁺通道阻断剂(Verapamil、LaCl₃)进行处理,研究在菌丝吸收过程中Cd²⁺与Ca²⁺以及Ca²⁺通道之间的关系.试验结果表明,高Cd²⁺和LaCl₃处理可促进菌丝发育;高Cd²⁺和Verapamil、LaCl₃处理不同程度地降低了菌丝对Ca²⁺的吸收;高Ca²⁺和LaCl₃处理则可提高菌丝对Cd²⁺的吸收.根据本试验结果可以认为,Cd²⁺在菌丝上的跨膜转运不是通过Ca²⁺通道完成,而且高Ca²⁺水平有利于Cd²⁺被菌丝吸收.

• Abstract：Arbuscular mycorrhizal fungi (AMF) in combination with phytoremediation have been regarded as an effective strategy in resolving the increasingly serious heavy metal (HM) contamination problem. However,the mechanisms underlying the uptake of HM by AMF hyphae are still unclear. In the present study,clover (Trifolium repense L.) and Glomus intraradices were taken as host plant and AM Fspecies,respectively. The extraradical hyphae and the roots were separated using a compartmented system. After Cd²⁺,Ca²⁺ and Ca²⁺ channel blockers (verapamil or LaCl₃) were added into the hyphal compartment,the uptake of Cd²⁺ and Ca²⁺ by hyphae and their relations with the Ca²⁺ channel were explored. The results indicated that high levels of Cd²⁺ and LaCl₃ increased the hyphal development. High levels of Cd²⁺,verapamil and LaCl₃ decreased the uptake of Ca²⁺ by hyphae to different degrees,while high levels of Cd²⁺ and LaCl₃ promoted the uptake of Cd²⁺ by hyphae. It is suggested that the transmembrane transport of Cd²⁺ is not achieved via the Ca²⁺ channel,and furthermore a high level of Ca²⁺ is beneficial to the uptake of Cd²⁺ by hyphae.

• Key words：arbuscular mycorrhizal fungi (AMF)  cadmium  calcium  calcium ion channel blockers  clover (Trifolium repense L.)

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