污染控制技术及原理
李慧芬,林雁冰,王娜娜,郭军康,韦革宏.一株Zn抗性菌株的筛选鉴定及吸附条件优化[J].环境科学学报,2010,30(11):2189-2196
一株Zn抗性菌株的筛选鉴定及吸附条件优化
- Screening and identification of a heavy metal-resistant strain and optimization of its zinc biosorption conditions
- 基金项目：国家自然科学基金(No30670372,30970003,30900215)
- 作者
- 单位
- 李慧芬
- 西北农林科技大学生命科学学院, 陕西省农业分子生物学重点实验室, 杨凌 712100
- 林雁冰
- 西北农林科技大学生命科学学院, 陕西省农业分子生物学重点实验室, 杨凌 712100
- 王娜娜
- 西北农林科技大学生命科学学院, 陕西省农业分子生物学重点实验室, 杨凌 712100
- 郭军康
- 西北农林科技大学生命科学学院, 陕西省农业分子生物学重点实验室, 杨凌 712100
- 韦革宏
- 西北农林科技大学生命科学学院, 陕西省农业分子生物学重点实验室, 杨凌 712100
- 摘要：采用浓度梯度法从铅锌尾矿区与污水灌溉区土壤中筛选了一株Zn抗性放线菌,并通过形态与培养特征、生理生化特性、细胞壁组分与16SrRNA基因序列分析对该菌株进行了鉴定.同时,综合运用单因素试验与正交试验法对该菌株吸附Zn2+的条件进行了优化.结果表明,菌株CCNWHX72-14经鉴定为高加索链霉菌(Streptomycesciscaucasicus),其对Zn2+的抗性达到845mg·L-1.锌胁迫生长曲线表明,该菌株在130mg·L-1Zn2+胁迫时生长良好,且最适培养时间为7d.各个单因素条件对该菌株吸附Zn2+的影响顺序为:接种量>初始Zn2+浓度>初始pH>转速>温度.该菌株吸附Zn2+的最佳条件为:初始Zn2+浓度为150mg·L-1,初始pH=5,接种量为1%,转速为60r·min-1,温度为28℃.菌株CCNWHX 72-14在最佳条件下对Zn2+的吸附较具有良好的重复性与稳定性,最高吸附量可达51.05mg·g-1,该研究为进一步探讨链霉菌的Zn2+吸附机制及其在生物修复中的应用奠定了基础.
- Abstract：We screened actinomycetes with heavy metal resistance and optimize the biosorption conditions of Zn2+ using concentration gradient methods.Morphological and cultural characteristics,physiological and biochemical properties,cell-wall pattern,and 16Sr RNA gene sequence were used for identification.Aheavy metal-resistant strain,designated CCNWHX 72-14,with resistance to 845 mg·L-1 of Zn2+,was isolated and identified as Streptomyces ciscaucasicu.It grew well in the presence of 130 mg·L-1 Zn2+ and the optimum culture time was 7 d.Both single factor and orthogonal experiments were designed to determine the optimum conditions for zinc biosorption and the order of each factors'effect was:inoculation dose > initial Zn2+ concentration > initial pH > agitation speed > temperature.The optimum conditions for zinc biosorption were:initial Zn2+ concentration 150 mg·L-1,initial pH 5,1% inoculation dose,60 r·min-1 and 28 ℃.The strain grew well with good repeatability and stability under the optimum conditions,and the maximum biosorption capacity of CCNWHX 72-14 under the experimental conditions reached 51.05 mg·g-1.The present research has laid a foundation for further investigation into the zinc biosorption mechanism of this strain and its application in environmental bioremediation.
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