• 关键词：内源过程  细胞衰减  细胞死亡  活性衰减  磷酸盐释放速率(PRR)  荧光原位杂交(FISH)  LIVE/DEAD染色

• 基金项目：国家自然科学基金项目(No.50678017);北京市属高等学校人才强教-深化计划高层次人才项目(No.PHR20100508)

• 王啟林
• 北京建筑工程学院, 城市雨水系统与水环境省部共建教育部重点实验室/可持续环境生物技术研发中心, 北京 100044

• 郝晓地
• 北京建筑工程学院, 城市雨水系统与水环境省部共建教育部重点实验室/可持续环境生物技术研发中心, 北京 100044

• 曹亚莉
• 北京建筑工程学院, 城市雨水系统与水环境省部共建教育部重点实验室/可持续环境生物技术研发中心, 北京 100044

• 摘要：通过磷酸盐释放速率(PRR)测定、荧光原位杂交技术(FISH)及LIVE/DEAD细胞染色技术,分别研究了生物营养物去除(BNR)系统与富集聚磷菌(PAOs)序批式反应器(SBR)系统中PAOs在好氧环境下的衰减特征.结果表明,当富集聚磷菌SBR系统进料中碳源(三水合乙酸钠和丙酸)是以36d为一个循环周期方式投加时,即三水合乙酸钠24d和丙酸12d,系统中PAOs富集比例可达91%.测定与计算结果表明,生物营养物去除(BNR)系统与富集聚磷菌SBR系统中PAOs衰减速率分别为0.113d^-1和0.181d^-1,死亡速率分别为0.048d^-1和0.036d^-1.这说明由细胞死亡引起的PAOs数量衰减在两个系统中分别占细胞总衰减的42%(BNR)和20%(SBR),而由细胞活性降低引起的活性衰减分别占细胞总衰减的58%(BNR)和80%(SBR).由此可见,PAOs数量衰减在其细胞总衰减中只占较小一部分,而绝大部分衰减是由活性衰减所引起.

• Abstract：By means of measuring maximal anaerobic phosphate release rates(PRR),analyzing 16 Sr RNA with fluorescence in-situ hybridization(FISH) and observing membrane integrity by live/dead staining,the aerobic decay characteristics of polyphosphate-accumulating organisms(PAOs) in a biological nutrient removal(BNR) system and an enriched PAOs sequencing batch reactor(SBR) system were investigated.It was experimentally determined that a highly enriched culture of PAOs(91%) was obtained in the enriched PAO-SBRsystem by alternating the carbon source in the feed between sodium acetate trihydrate and propionate over a 36 d switching cycle,i.e.,sodium acetate trihydrate(24 d) and propionate(12 d).The experimental results and calculations revealed that the decay rates of PAOs in the BNRand enriched PAO-SBRsystems were 0.113 d^-1 and 0.181 d^-1,respectively,and the death rates were 0.048 d^-1 and 0.036 d^-1.In addition,the results also demonstrated that cell death contributed 42% and 20%,respectively,to the cell decay in the BNRand enriched PAO-SBRsystems.In other words,the activity decay accounted for 58% and 80% of the total cell decay.For this reason,cell death was not a major factor causing the cell decay of PAOs,but activity decay was mostly responsible for this process.

• Key words：endogenous processes  cell decay  cell death  activity decay  phosphate release rate(PRR)  fluorescence in-situ hybridization(FISH)  LIVE/DEAD staining

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