研究报告
杨婷,林先贵,胡君利,张晶,吕家珑,王一明,王俊华.发酵牛粪和造纸干粉对多环芳烃污染土壤菌根修复的影响[J].环境科学学报,2011,31(1):144-149
发酵牛粪和造纸干粉对多环芳烃污染土壤菌根修复的影响
- Effects of fermented cow dung and dry powdered sludge from papermaking waste on mycorrhizoremediation of PAH-contaminated soils
- 基金项目:国家高技术研究发展计划(863)项目(No.2007AA061101);国家自然科学基金项目(No.40801091)
- 杨婷
- 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室, 南京 210008; 2. 西北农林科技大学资源环境学院, 杨凌 712100
- 林先贵
- 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室, 南京 210008; 2. 中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室, 南京 210008
- 胡君利
- 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室, 南京 210008; 2. 中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室, 南京 210008
- 张晶
- 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室, 南京 210008; 2. 中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室, 南京 210008
- 吕家珑
- 西北农林科技大学资源环境学院, 杨凌 712100
- 王一明
- 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室, 南京 210008; 2. 中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室, 南京 210008
- 王俊华
- 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室, 南京 210008; 2. 中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室, 南京 210008
- 摘要:通过温室盆栽试验,研究了添加发酵牛粪和造纸干粉对菌根化紫花苜蓿修复多环芳烃(PAHs)污染农田土壤的影响.结果发现,添加0.5%~2.0%发酵牛粪对紫花苜蓿根系丛枝菌根(AM)真菌侵染率无明显影响,但均显著提高了紫花苜蓿的植株生物量(p<0.05),其中,添加1.0%和2.0%发酵牛粪处理的土壤PAHs含量较对照略有降低.添加0.05%和0.1%造纸干粉均显著提高了紫花苜蓿的AM真菌侵染率和植株生物量(p<0.05),但添加0.2%造纸干粉却对紫花苜蓿的AM真菌侵染率和植株生物量产生了显著的抑制作用(p<0.05),仅添加0.05%造纸干粉处理的土壤PAHs含量显著低于对照(p<0.05),且3~5环PAHs降解率均得到显著提高(p<0.05).此外,土壤中PAHs降解率与AM真菌侵染率之间呈线性回归关系(R2=0.247,F=6.217,p<0.05).以上结果表明,添加适量(0.5%~2%)发酵牛粪可通过直接增加养分供应来促进植物生长,但对PAHs降解影响较小;添加微量(0.05%~0.1%)造纸干粉可通过增进AM真菌侵染来促进植株生长,加速PAHs降解,因而可作为刺激性物质应用于菌根修复.
- Abstract:Agreenhouse pot-experiment was carried out to investigate the effects of fermented cow dung(FD) and dry powdered sludge of papermaking waste(PP) on phytoremediation of PAH-contaminated farmland soils by arbuscular mycorrhizal fungi(AMF) infected alfalfa(Medicago sativa L.). The addition of 0.5% to 2.0% FDhad no significant effect on AMFinfection rate,but could promote growth of alfalfa(p<0.05). PAHresidues in the 1.0% and 2.0% FDtreatments were lower than those in the control treatment. The addition of 0.05% and 0.1% PPenhanced AMFinfection rate and plant biomass significantly(p<0.05),while a negative effect was observed for the 0.2% treatment(p<0.05). Significant degradation of PAHresidues was observed only from the treatment with 0.05% PP(p<0.05),and the degradation rates of PAHs with three,four or five-ring were higher than those of the control(p<0.05). Alinear regression relation was observed between PAHdegradation rate in soil and the AMFinfection rate in plant roots(R2=0.247,F=6.217,p<0.05). These results indicated that the appropriate amount(0.5%~2%) of FDaddition could increase nutrition to promote plant growth,but had little effect on the degradation of PAHs. However,small amounts(0.05%~0.1%) of PPaccelerated AMFinfection to improve plant growth and the degradation of PAHs,suggesting that it could be used as a stimulant in mycorrhizoremediation of PAH-contaminated soils.
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