• 关键词：微囊藻种群丰度  产毒微囊藻  荧光定量PCR  太湖

• 基金项目：国家重点基础研究发展计划(No.2008CB418000);中科院南京地理与湖泊研究所所长基金(No.07SL021001)

• 李大命
• 1. 中国科学院南京地理与湖泊研究所, 湖泊与环境国家重点实验室, 南京 210008; 2. 中国科学院研究生院, 北京 100049

• 孔繁翔
• 中国科学院南京地理与湖泊研究所, 湖泊与环境国家重点实验室, 南京 210008

• 于洋
• 中国科学院南京地理与湖泊研究所, 湖泊与环境国家重点实验室, 南京 210008

• 张民
• 中国科学院南京地理与湖泊研究所, 湖泊与环境国家重点实验室, 南京 210008

• 史小丽
• 中国科学院南京地理与湖泊研究所, 湖泊与环境国家重点实验室, 南京 210008

• 摘要：运用实时荧光定量PCR(Quantitative real-timePCR,QPCR)技术研究了太湖蓝藻水华期间不同湖区水体和底泥中产毒微囊藻与总微囊藻种群丰度.结果表明,湖区间产毒微囊藻种群和总微囊藻种群丰度明显不同:在水体中,竺山湾(N5)和梅梁湾(N2)产毒微囊藻和总微囊藻种群丰度高于贡湖湾(N4)和湖心(S4).同时发现,产毒微囊藻占总微囊藻种群比例在湖区间存在差异:竺山湾与梅梁湾产毒微囊藻占总微囊藻种群比例较高,分别为41.21%和40.25%,贡湖湾和湖心产毒微囊藻占总微囊藻种群比例分别为28.53%和9.36%.底泥中产毒微囊藻和总微囊藻种群丰度在湖区间存在差异,竺山湾、梅梁湾、贡湖湾和湖心底泥样品中产毒微囊藻占总微囊藻种群的比例分别为6.51%、8.49%、5.14%和2.22%.从以上研究结果可以得出,在太湖蓝藻水华暴发期间,水体和底泥中微囊藻种群丰度及其组成存在空间差异,富营养化水平高的湖区产毒微囊藻和总微囊种群丰度均高于富营养化水平低的湖区,产毒微囊藻占总微囊藻种群的比例也较高.在同一采样点,底泥中产毒微囊藻占总微囊藻种群比例低于水体.

• Abstract：The abundances of microcystin-producing and total Microcystis populations in the water column and surface sediment in Lake Taihu during water bloom were investigated by using quantitative real-time PCR (QPCR). Results showed that microcystin-producing and total Microcystis,in both the water column and sediment,were more abundant in Zhushan Bay and Meiliang Bay than in Gonghu Bay and the center of the lake. The proportion of toxic Microcystis species to the total Microcystis populations in water column from Zhushan Bay,Meiliang Bay,Gonghu Bay,and the center of the lake were 41.12%,40.25%,28.53%,and 9.36%,respectively. In sediment,toxic Microcystis species accounted for 6.51%,8.49%,5.14%,and 2.22% of the total Microcystis populations in those four lake regions,respectively. In conclusion,the abundance and composition of Microcystis differed spatially in the water column and surface sediment in Lake Taihu during the water bloom. The abundances of toxic and total Microcystis populations in the water column and sediment are relatively high in the high trophic lake regions and,in parallel,the proportion of toxic Microcystis to total Microcystis population is also high when the water body has a high trophic level. In addition,toxic Microcystis occupied much more of the total Microcystis in the water column than in sediment.

• Key words：the abundance of Microcystis population  microcystin-producing Microcystis  quantitative real-time PCR  Lake Taihu

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