• 关键词：染料  脱色  锰过氧化物酶(MnP)  酶活  混合培养  生物吸附

• 基金项目：国家自然科学基金项目(No.30800030);山西省青年科技基金(No.207021030);山西省自然基金(No.2007031003)

• 杨秀清
• 山西大学生物技术研究所, 化学生物学与分子工程教育部重点实验室, 太原 030006

• 王婧人
• 山西大学生物技术研究所, 化学生物学与分子工程教育部重点实验室, 太原 030006

• 赵晓霞
• 山西大学生物技术研究所, 化学生物学与分子工程教育部重点实验室, 太原 030006

• 薛瑞
• 山西大学生物技术研究所, 化学生物学与分子工程教育部重点实验室, 太原 030006

• 摘要：利用本实验室新构建的白腐菌Trametes sp.SQ01和毛壳菌Chaetomium sp.R01混合培养体系,对刚果红、酸性红、橙黄G和溴酚蓝4种染料进行了脱色研究.结果表明,SQ01与R01混合培养所产生的锰过氧化物酶(MnP)酶活比菌株SQ01单独培养时提高了约5.5倍.菌株R01的接种量、接种时间对混合培养中MnP的酶活有很大影响,菌株R01接种量过多或过少,都会使混合培养产生的MnP酶活降低.随着R01接种时间的延迟,MnP酶活也逐渐降低.4种染料对SQ01与R01混合培养时MnP的酶活有抑制作用,酶活大约降低了40%～75%,其中,酸性红对MnP酶活的抑制作用最强.菌株SQ01与R01的混合培养对4种染料的脱色率高于SQ01单一菌株的脱色率,提高了约20%～50%.混合培养第3d,SQ01与R01混合培养体系对刚果红、酸性红、橙黄G和溴酚蓝的脱色率分别为90%、75%、76%、94%,而菌株SQ01单独培养时的脱色率分别只有75%、43%、41%、63%.染料的加入时间对脱色有影响,混合培养4d后加入染料的脱色率高于混合培养0d后加入染料的脱色率.在4种染料的脱色过程中,除了生物降解作用外,生物吸附也起着重要作用.混合培养对橙黄G、溴酚蓝和酸性红的生物吸附率分别为2%、5%和15%,而对刚果红的生物吸附率高达60%.利用真菌混合培养对染料进行脱色研究,其高效的脱色能力表明该混合培养体系在染料脱色处理方面有着广阔的应用前景.

• Abstract：Four dyes including Congo red,acid red,orange Gand bromphenol blue were decolorized by a mixed fungal culture of Trametes sp. SQ01 and Chaetomium sp. R01,which were newly developed in our laboratory.The results indicated that MnPactivity in the mixed culture of strain SQ01 and R01 increased approximately 5.5 times over that obtained from a pure culture of SQ01.The amount and timing of strain R01 had a marked effect on MnPproduction by SQ01.If the inoculum of strain R01 was too high or too low,the MnPactivity decreased in the mixed culture.As the inoculation time of strain R01 was delayed,MnPactivity was gradually reduced.MnPproduction was inhibited by about 40%～75% by the four dyes in mixed culture of SQ01 and R01.The decolorization rates of mixed culture of strain SQ01 and R01 were increased by 20%～50% compared to that of the monoculture of SQ01.After 3 days,75%～94% of four dyes were decolorized by mixed culture of SQ01 and R01,while the decolorization rate was only about 41%～75% by strain SQ01 cultured alone.The time-point of dye addition remarkably influenced the efficiency of decolorization by the mixed culture.The decolorization rates of dyes added to the medium after 4 days of mixed cultivation were significantly higher as compared to dyes added at the outset of mixed cultivation.In addition to biodegradation,biosorption also played an important role in the decolorization process.The biosorption of orange G,bromphenol blue and acid red accounted for 2%,5% and 15% of the total color removal,respectively.However,the biosorption rate of congo red reached up to 60%.To date,this is the first report of decolorizing dyes by mixed fungal cultures. The high efficiency of the decolorization ability indicates that the mixed culture has broad application prospects in dye decolorization treatment.

• Key words：dyes  decolorization  MnP  activity  mixed culture  biosorption

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