• 关键词：文昌鱼  六溴环十二烷(HBCD)  酸性磷酸酶(ACP)  碱性磷酸酶(ALP)  过氧化氢酶(CAT)  超氧化物歧化酶(SOD)

• 基金项目：国家高技术研究发展计划项目(No2008AA092603);环境化学与生态毒理学国家重点实验室开放课题(NoKF2010-14)

• 王玲
• 青岛大学化学化工与环境学院, 青岛 266071

• 郑明刚
• 国家海洋局第一海洋研究所海洋生态中心, 青岛 266061

• 仝艳丽
• 国家海洋局第一海洋研究所海洋生态中心, 青岛 266061

• 李海东
• 国家海洋局第一海洋研究所海洋生态中心, 青岛 266061

• 孙修勤
• 国家海洋局第一海洋研究所海洋生态中心, 青岛 266061

• 摘要：以文昌鱼为研究对象,研究了在六溴环十二烷暴露下对文昌鱼造成的机体氧化损伤.通过检测相关酶活变化情况,如酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD),从而确定低剂量长期暴露HBCD对文昌鱼鱼体产生氧化损伤效应的机制.急性毒性实验结果表明,文昌鱼暴露于六溴环十二烷饱和溶液(8.6ng·mL^-1) 96h后未有死亡发生,说明该物质毒性相对较小;亚慢性毒性试验表明,梯度暴露实验设置浓度分别为1.00、2.15、4.30、8.60ng·mL^-1,暴露时间为14d,按体长将处于Ⅱ龄(2.0～2.8cm)和Ⅲ龄(3.2～4.0cm)的文昌鱼分别投毒检测,实验结果与对照组比较:Ⅱ龄文昌鱼的ACP、ALP、SOD活性均在1ng·mL^-1浓度范围内受促进作用显著,2.15ng·mL^-1时受抑制作用显著,然后随浓度升高酶活升高.CAT在2.15ng·mL^-1时受促进作用显著,当浓度大于2.15ng·mL^-1时受抑制作用显著,但并无明显剂量关系.Ⅲ龄文昌鱼总体上酶活变化较小,也无剂量关系.结果表明HBCD对Ⅱ龄文昌鱼的4种酶活影响比Ⅲ龄显著,与对照组相比,所有暴露组文昌鱼体重增长缓慢(p<0.05).

• Abstract：The objective of this study was to evaluate the sub-lethal toxicity of the fire retardant hexabromocyclododecane(HBCD) in fish.Amphioxuses,as in vivo models,were exposed to waterborne HBCDfrom 1 to 8.6 ng·mL^-1 for 14 days.Acid phosphatase(ACP),alkaline phosphatase(ALP),catalase(CAT),and superoxide dismutase(SOD) activities were measured.The results of acute toxicity experiments show that there were no amphioxus deaths after 96 h exposure in saturated solution of HBCD(8.6 ng·mL^-1).The gradient exposure concentrations of the subchronic toxicity experiment were set at 1.00,2.15,4.30,8.60 ng·mL^-1,and exposure time was 14 days.Age Ⅱ(2.0～2.8 cm) and age Ⅲ(3.2～4.0 cm) amphioxus were poisoned and detected separately.Compared to the control group,ACP,ALP,and SODof age Ⅱ are promoted significantly at a concentration of 1 ng·mL^-1,and inhibited significantly at 2.15 ng·mL^-1;while in the case of CAT,significant promotion is detected at a concentration of 2.15 ng·mL^-1.When the concentration is higher than 2.15ng·mL^-1,significant inhibition is observed.However,no visible relationship was found between dose and response.To the age Ⅲ,there are no significant changes for all the enzymes,which indicates that the effect of HBCDon age Ⅱ is significantly higher than on age Ⅲ.In addition,body weight increases more slowly compared to the control group(p<0.05).

• Key words：hexabromocyclododecane(HBCD)  amphioxus  acid phosphatase  alkaline phosphatase  catalase  superoxide dismutase

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