研究报告
戴灵鹏,董新姣,马海虎,盛丽梅.镉诱导满江红(Azolla imbricata)花色素苷的初步鉴定、抗氧化活性及其作用机理[J].环境科学学报,2011,31(5):1092-1097
镉诱导满江红(Azolla imbricata)花色素苷的初步鉴定、抗氧化活性及其作用机理
- Preliminary identification,antioxidant activity and mechanism of anthocyanins from Azolla imbricata induced by cadmium
- 基金项目：国家自然科学基金项目(No30800159)
- 作者
- 单位
- 戴灵鹏
- 温州大学生命与环境科学学院, 温州 325035
- 董新姣
- 温州大学生命与环境科学学院, 温州 325035
- 马海虎
- 温州大学生命与环境科学学院, 温州 325035
- 盛丽梅
- 温州大学生命与环境科学学院, 温州 325035
- 摘要：为了研究镉诱导的满江红花色素苷的分子结构,分别用0.1mg·L-1Cd、0.1%盐酸-甲醇溶液、XAD-7大孔树脂和葡聚糖凝胶SephadexLH-20柱对其进行诱导、提取、纯化和分离.经紫外可见光谱特性和HPLC图谱分析,初步确定此物质为木犀草定-5-葡萄糖苷.采用不同的体外实验方法评价了满江红花色素苷纯化前后的抗氧化活性.结果表明,花色素苷的总抗氧化能力、还原力、清除O2·-和·OH能力随花色素苷浓度的增加而增加,且纯化后的值大于纯化前.当花色素苷的浓度为0.5mg·mL-1时,纯化后的花色素苷的总抗氧化能力、还原力、清除O2·-和·OH能力分别为纯化前的3.16,1.36,2.71和1.86倍,证明木犀草定-5-葡萄糖苷是满江红抗氧化作用的主要物质基础.此外,本文还对花色素苷的抗氧化机理进行了探讨.总的来说,木犀草定-5-葡萄糖苷的合成是满江红抗性机理之一,可缓解镉的毒害.
- Abstract：In order to study the molecular structure of the anthocyanins which are induced by Cd in Azolla imbricata,anthocyanins were induced with 0.1 mg·L-1 Cd,extracted with 0.1% HCl in methanol,purified by an Amberlite XAD-7 column and separated by Sephadex LH-20 column.The main compound was preliminarily identified as luteolinidin-5-glucoside by its ultraviolet/visible absorbance spectrum and high performance liquid(HPLC) chromatography.In vitro antioxidant properties of anthocyanins before and after purification were also evaluated by different analytical methods.The results showed that the total antioxidant capacity,reducing power,scavenging activity of superoxide anion radical(O2·-)and hydroxyl radical(·OH)increased significantly with increasing concentrations of anthocyanin extracts,and the antioxidant activity of anthocyanins after purification was higher than the values of anthocyanins before purification.When the concentration of anthocyanins reached 0.5 mg·mL-1,the values of anthocyanins after purification were increased by 3.16,1.36,2.71 and 1.86 times respectively,compared to anthocyanins before purification,indicating that luteolinidin-5-glucoside was the most important substance responsible for the antioxidant properties of A. imbricata.In addition,the antioxidant mechanism of anthocyanins is also discussed in this paper.In conclusion,the results obtained in this work indicated that the increase in the content of anthocyanins may be involved in internal detoxification mechanisms of A. imbricata against Cd toxicity.
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