• 关键词：复合生物滤池  老龄渗滤液  通气  污染物去除  微生物群落结构  PCR-DGGE

• 基金项目：国家自然科学基金资助项目(No.20977031);上海市自然科学基金资助项目(No.09ZR1409000)

• 戴淑萍
• 华东师范大学环境科学系, 上海市城市化生态过程与生态恢复重点实验室, 上海 200062

• 谢冰
• 华东师范大学环境科学系, 上海市城市化生态过程与生态恢复重点实验室, 上海 200062

• 李斐斐
• 华东师范大学环境科学系, 上海市城市化生态过程与生态恢复重点实验室, 上海 200062

• 吕宝一
• 华东师范大学环境科学系, 上海市城市化生态过程与生态恢复重点实验室, 上海 200062

• 唐媛
• 华东师范大学环境科学系, 上海市城市化生态过程与生态恢复重点实验室, 上海 200062

• 摘要：以上海老港垃圾填埋场填埋多年的陈垃圾和废弃多孔滤料为填料构建复合生物滤池处理晚年期垃圾渗滤液.研究了不同运行条件下复合生物滤池对晚年期渗滤液的处理效果,并对不同通气状况下功能微生物的数量、组成和群落结构,以及与处理效果之间的关系进行了分析.结果表明:通气对污染物的去除率有较明显的提高,在复合生物滤池中部通气条件下(气固比0.3)较未通气复合生物滤池对COD、NH₄⁺-N和TN的去除率分别高出10、30和10个百分点,而继续增大通气(气固比0.4)对污染物质的去除增加不明显.微生物计数结果表明,复合生物滤池上部微生物数量明显多于下部,上部的异样细菌和硝化细菌数量明显增多,亚硝化细菌数量明显减少,反硝化细菌变化不显著.PCR-DGGE结果表明通气较未通气条带数量和亮度有明显的改变,说明通气改变了反应器中的微生物群落结构.测序结果表明滤池中主要的优势微生物与Pseudomonas sp. HPC1354、Alcanivorax sp. 521-1、uncultured β-proteobacterium、Dechloromonas aromatica RCB、Proteobacteria.等具有很高的相似性.

• Abstract：Two different media, aged refuse and waste porous filter material, were used to construct composite biofilters. Their treatment efficiencies on mature landfill leachate pollutants under different operating conditions were investigated. The microbial number, composition and structure of functional micro-organism under different aeration conditions, and their relationship with the treatment effects were analyzed. The results showed that the aeration could enhance the pollutant removal significantly. COD, NH₄⁺-N and the TN removal rates with aeration were respectively 10%, 30% and 10% higher than without aeration. Additional aeration had no further influence on pollutant removal. Micro-organism counting showed that the number of bacteria at the top of both aerated and non-aerated stages were higher than that at the bottom. In the upper part, the number of heterotrophic bacteria and nitrifying bacteria significantly increased, nitrite bacteria significantly reduced, and denitrifying bacteria did not change much. Obvious changes in both the number and brightness of the sequencing results of PCR-DGGE bands from aeration to non-aeration indicated that the structure of the microbial community is closely related with the aeration. The result also showed that the major microbial colonies in the bioreactor had high similarity with Proteobacteria and others.

• Key words：composite biofilter  mature landfill leachate  aeration  pollutant removal  microbial community structure  PCR-DGGE

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